Hantschmann Peer, Beysiegel Sonja, Assemi Cyamak, Kurzl Rainer
First Frauenklinik, Klinikum Innenstadt, Ludwig Maximilians University-Munich, Munich, FRG.
J Reprod Med. 2004 Oct;49(10):787-95.
To analyze nm23-H1 protein immunohistologically in squamous cell carcinoma of the vulva.
In 68 carcinomas, tumor type, grade, inflammation and vascular space involvement were determined. Formalin-fixed, paraffin-embedded tissue was stained for nm23-H1. Staining pattern, intensity and percentage of stained tumor cells were evaluated. Staining results were analyzed statistically univariately by the chi2 test, Kaplan-Meier survival analysis with log-rank test and receiver operator characteristic curves and multivariately by proportional hazard analysis.
Of the tumors, 9% were negative for nm23-H1. Positive tumors showed 4 staining patterns. Tumors with inhomogeneous expression of nm23-H1 tended to have shorter disease-free and overall survival. Carcinomas with nm23-H1 staining in <50% of the tumor cells more frequently developed nonlocal tumor recurrences (p= 0.018) and showed shorter disease-free survival (p = 0.017). Tumors with high nm23-H1 expression were associated with patients younger than 60 years (p = 0.02).
Immunohistologic staining for nm23-H1 protein shows different staining patterns and percentages of stained cells in squamous cell carcinoma of the vulva. Tumors with inhomogeneous and low nm23-H1 expression are associated with a worse prognosis.
对外阴鳞状细胞癌进行nm23-H1蛋白的免疫组织学分析。
对68例癌组织确定肿瘤类型、分级、炎症及血管间隙受累情况。用福尔马林固定、石蜡包埋的组织进行nm23-H1染色。评估染色模式、强度及染色肿瘤细胞的百分比。染色结果采用卡方检验进行单因素统计学分析、用对数秩检验的Kaplan-Meier生存分析及受试者工作特征曲线分析，并采用比例风险分析进行多因素分析。
在这些肿瘤中，9%的肿瘤nm23-H1呈阴性。阳性肿瘤呈现4种染色模式。nm23-H1表达不均匀的肿瘤无病生存期和总生存期往往较短。肿瘤细胞中nm23-H1染色<50%的癌更常发生非局部肿瘤复发（p = 0.018），且无病生存期较短（p = 0.017）。nm23-H1高表达的肿瘤与年龄小于60岁的患者相关（p = 0.02）。
nm23-H1蛋白的免疫组织学染色在外阴鳞状细胞癌中显示出不同的染色模式和染色细胞百分比。nm23-H1表达不均匀和低表达的肿瘤预后较差。
