Zhang Zhen-lin, Qin Yue-juan, Huang Qi-ren, He Jin-wei, Li Miao, Zhou Qi, Hu Yun-qin, Li Yu-juan
Center for Preventing and Treating Osteoporosis, Osteoporosis Research Unit, The Sixth People Hospital, Shanghai Jiaotong University, Shanghai 200233, China.
Acta Pharmacol Sin. 2004 Dec;25(12):1690-7.
To investigate the correlation between calcium treatment in postmenopausal women and estrogen receptor-alpha (ER-alpha) Xba I and Pvu II genotype and vitamin D receptor (VDR) Apa I genotype.
One hundred fifteen postmenopausal Chinese women of Han population were enrolled and treated with calcichew-D3 (1000 mg calcium and 400 U vitamin D3) daily for 1 year. At entry and after 1 year treatment, the bone mineral density (BMD), serum and urinary bone turnover biochemical markers were evaluated. ER-alpha and VDR genotype were analyzed using PCR-restriction fragment length polymorphism.
After 1 year of calcium supplementation, a significant increase of BMD and a marked reduction in serum ALP and PTH levels, and a significant increase of serum 25-(OH) vitamin D level were observed (P<0.01 or P<0.05). At entry and after 1 year of treatment, no significant association was found between Xba I, Pvu II, and Apa I genotypes and BMD in L1-4, Neck, and Troch, and all bone turnover marker levels. However, the percentage of change (median, QR) in Neck BMD was significantly different in homozygous XX [-4.14 (from -6.54 to -1.34)] in comparison with Xx [1.72 (from -1.12 to 3.20)] (P<0.001) or xx [1.22 (from -1.74 to 3.06)] Xba I ER-alpha genotype (P=0.001).
Women with ER-alpha Xba I genotype XX may have a higher risk of relatively fast bone mass loss in femoral neck after menopause and that they may have a poor responsiveness to calcium supplementation. The changes in BMD are not associated with ER-alpha Pvu II genotype and VDR Apa I genotype after 1 year of calcium supplementation.
探讨绝经后女性钙治疗与雌激素受体α（ER-α）Xba I和Pvu II基因型以及维生素D受体（VDR）Apa I基因型之间的相关性。
纳入115名汉族绝经后中国女性，每日给予钙尔奇-D3（1000mg钙和400U维生素D3）治疗1年。在入组时和治疗1年后，评估骨密度（BMD）、血清和尿骨转换生化标志物。采用聚合酶链反应-限制性片段长度多态性分析ER-α和VDR基因型。
补钙1年后，观察到BMD显著增加，血清碱性磷酸酶（ALP）和甲状旁腺激素（PTH）水平显著降低，血清25-（OH）维生素D水平显著升高（P<0.01或P<0.05）。在入组时和治疗1年后，未发现Xba I、Pvu II和Apa I基因型与L1-4、颈部和粗隆部的BMD以及所有骨转换标志物水平之间存在显著关联。然而，与Xx [1.72（-1.12至3.20）] [P<0.001]或xx [1.22（-1.74至3.06）] Xba I ER-α基因型相比，颈部BMD的变化百分比（中位数，四分位数间距）在纯合子XX [-4.14（-6.54至-1.34）]中显著不同（P=0.001）。
ER-α Xba I基因型为XX的女性在绝经后股骨颈骨量相对快速丢失的风险可能更高，且她们可能对补钙反应较差。补钙1年后，BMD的变化与ER-α Pvu II基因型和VDR Apa I基因型无关。
