Savoia D, Biglino S, Cestaro A, Zucca M
Institute of Microbiology, Torino, Italy.
Microbiologica. 1992 Jan;15(1):51-6.
Uptake of Leishmania major and Leishmania infantum promastigotes by U937 cells was determined by microscopic observation and by using radiolabelled parasites. With both species we observed an increase in uptake after parasite pretreatments with fibronectin, and a decrease in uptake after cell pretreatment with interferon-gamma (IFN gamma). When both pretreatments were performed, the uptake was significantly decreased only in L. infantum experiments. These findings may be of some importance in the evaluation of IFN gamma as a leishmanicidal agent.
通过显微镜观察和使用放射性标记的寄生虫，确定了U937细胞对硕大利什曼原虫和婴儿利什曼原虫前鞭毛体的摄取情况。对于这两种利什曼原虫，我们观察到用纤连蛋白预处理寄生虫后摄取增加，而用γ干扰素（IFNγ）预处理细胞后摄取减少。当进行这两种预处理时，仅在婴儿利什曼原虫实验中摄取显著降低。这些发现可能在评估IFNγ作为杀利什曼原虫剂方面具有一定重要性。
