Egertsdotter U, van Zyl L M, MacKay J, Peter G, Kirst M, Clark C, Whetten R, Sederoff R
Georgia Institute of Technology, Institute of Paper Science and Technology, 500 10th Street, Atlanta, GA 30332, USA.
Plant Biol (Stuttg). 2004 Nov;6(6):654-63. doi: 10.1055/s-2004-830383.
The natural variability of wood formation in trees affords opportunities to correlate transcript profiles with the resulting wood properties. We have used cDNA microarrays to study transcript abundance in developing secondary xylem of loblolly pine (Pinus taeda) over a growing season. The cDNAs were selected from a collection of 75 000 ESTs that have been sequenced and annotated (http://web.ahc.umn.edu/biodata/nsfpine/). Cell wall thickness and climatic data were related to earlywood and latewood formation at different time points during the growing season. Seventy-one ESTs showed preferential expression in earlywood or latewood, including 23 genes with no significant similarity to genes in GenBank. Seven genes involved in lignin synthesis were preferentially expressed in latewood. The studies have provided initial insights into the variation of expression patterns of some of the genes related to the wood formation process.
树木中木材形成的自然变异性为将转录本谱与所得木材特性相关联提供了机会。我们使用cDNA微阵列研究了火炬松（Pinus taeda）在一个生长季节中发育中的次生木质部的转录本丰度。这些cDNA是从75000个已测序和注释的ESTs集合中挑选出来的（http://web.ahc.umn.edu/biodata/nsfpine/）。细胞壁厚度和气候数据与生长季节不同时间点的早材和晚材形成相关。71个ESTs在早材或晚材中表现出优先表达，其中包括23个与GenBank中的基因无显著相似性的基因。7个参与木质素合成的基因在晚材中优先表达。这些研究为与木材形成过程相关的一些基因的表达模式变化提供了初步见解。
