Lammi Mikko J, Qu Cheng-Juan, Laasanen Mikko S, Saarakkala Simo, Rieppo Jarno, Jurvelin Jukka S, Töyräs Juha
Department of Anatomy, University of Kuopio, 70211 Kuopio, Finland.
J Rheumatol. 2004 Dec;31(12):2449-53.
To test whether there is undersulfation of chondroitin sulfate in osteoarthritic bovine articular cartilage to support the hypothesis that sulfate deficiency is involved with the development of osteoarthritis.
Cartilage samples from bovine patellae (n = 32) were divided into 3 groups based on their osteoarthritic progression, as assessed by modified Mankin score. Uronic acid contents of the samples were determined. Fragmentation of the proteoglycans due to proteolytic processing was estimated with agarose gel electrophoresis. The molar ratios of chondroitin sulfate isoforms in the extracted proteoglycans were determined with fluorophore-assisted carbohydrate electrophoresis.
Loss of proteoglycans and accumulation of tissue water was evident in groups II and III, and progressive OA increased heterogeneity of aggrecan population in groups II and III. Importantly, the molar ratio of nonsulfated disaccharide was decreased in the osteoarthritic articular cartilage.
The structure of chondroitin sulfate in degenerated bovine cartilage did not support the hypothesis that sulfate depletion is present in osteoarthritic joint.
检测骨关节炎牛关节软骨中硫酸软骨素是否存在硫酸化不足，以支持硫酸盐缺乏与骨关节炎发展有关的假说。
根据改良曼金评分评估的骨关节炎进展情况，将取自牛髌骨的软骨样本（n = 32）分为3组。测定样本中糖醛酸含量。用琼脂糖凝胶电泳估计蛋白聚糖因蛋白水解加工导致的片段化。用荧光辅助碳水化合物电泳测定提取的蛋白聚糖中硫酸软骨素异构体的摩尔比。
在第II组和第III组中，蛋白聚糖丢失和组织水分积聚明显，并且进行性骨关节炎增加了第II组和第III组中聚集蛋白聚糖群体的异质性。重要的是，骨关节炎关节软骨中未硫酸化二糖的摩尔比降低。
退化牛软骨中硫酸软骨素的结构不支持骨关节炎关节中存在硫酸盐消耗的假说。
