Xue Jian-ping, Zhang Ai-ming, Gao Xiang, Sheng Wei
Department of Biology, Huaibei Coal Industry Teachers' College, Huaibei 235000, China.
Zhongguo Zhong Yao Za Zhi. 2004 Sep;29(9):834-7.
To select the sodium chloride tolerant mutants of the Chrysanthemum morifolium callus through tissue culture and EMS (ethylmethane sulfonate) treatment.
Calli were induced from the leaves of C. morifolium. The calli were treated with 0.2% and 0.5% EMS, respectively. After 15 days' culture, the calli were transplanted to selection media with 0.5%, 1.0% and 1.5% NaCl, and the sodium chloride tolerant mutants were selected out.
After tested the sodium chloride tolerant stability, the callus selected are found to be the mutants indeed.
通过组织培养和EMS（甲基磺酸乙酯）处理筛选出耐氯化钠的菊花愈伤组织突变体。
从菊花叶片诱导愈伤组织。将愈伤组织分别用0.2%和0.5%的EMS处理。培养15天后，将愈伤组织转移到含有0.5%、1.0%和1.5%氯化钠的选择培养基上，筛选出耐氯化钠的突变体。
经耐氯化钠稳定性测试，筛选出的愈伤组织确实是突变体。
