Fox James G, Rogers Arlin B, Whary Mark T, Taylor Nancy S, Xu Shilu, Feng Yan, Keys Susan
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Comp Med. 2004 Oct;54(5):571-7.
Although Helicobacter bilis infects mice worldwide, it is not known whether H. bilis causes enterohepatic disease in outbred Swiss Webster (SW) mice. Intestinal and liver specimens from four groups of 39 SW mice, five of which were treated with creatine in the drinking water, were obtained for culture for the presence of H. bilis and were analyzed as to whether infection status was associated with H. bilis seroconversion and/or hepatitis. Helicobacter bilis was isolated from the colon of all 27 mice of groups I-III, but only from the liver of one 12- to 13-month-old female mouse. Ten of 27 livers were H. bilis-positive based on polymerase chain reaction (PCR) analysis; 8 of 10 (80%) of the positive results were for older mice. Results of PCR analysis for H. bilis were negative, and H. bilis was not isolated from 12 control mice (group IV). Irrespective of treatment group or controls, severity of histologic lobular and periportal chronic inflammatory lesions in the liver of H. bilis-infected outbred mice ranged from minimally to moderately severe. Helicobacter bilis infection was associated with increased portal inflammation in group III mice, compared with age-matched, helicobacter-free, group IV mice (P < 0.03). A comparison of potential sex effects in group III mice indicated that H. bilis-infected female mice developed more severe portal inflammation than did H. bilis-infected male mice (P < 0.01). On the basis of results of an ELISA, 8 of 11, 6- to 8-month-old H. bilis-infected mice of group III seroconverted to H. bilis outer membrane antigen. Helicobacter bilis infection is associated with hepatitis in SW mice and can confound experimental results.
尽管胆汁螺杆菌在全球范围内感染小鼠，但尚不清楚胆汁螺杆菌是否会在远交系瑞士韦伯斯特（SW）小鼠中引起肝肠疾病。从四组共39只SW小鼠中获取肠道和肝脏标本，其中五只用含肌酸的饮用水处理，用于培养以检测是否存在胆汁螺杆菌，并分析感染状态是否与胆汁螺杆菌血清转化和/或肝炎相关。在I - III组的所有27只小鼠的结肠中均分离出胆汁螺杆菌，但仅在一只12至13月龄的雌性小鼠的肝脏中分离出该菌。基于聚合酶链反应（PCR）分析，27只肝脏中有10只为胆汁螺杆菌阳性；其中80%（8/10）的阳性结果来自年龄较大的小鼠。12只对照小鼠（IV组）的胆汁螺杆菌PCR分析结果为阴性，且未分离出胆汁螺杆菌。无论治疗组或对照组如何，胆汁螺杆菌感染的远交系小鼠肝脏中组织学小叶和汇管区慢性炎症病变的严重程度从轻度到中度不等。与年龄匹配、无螺杆菌的IV组小鼠相比，III组胆汁螺杆菌感染小鼠的门静脉炎症增加（P < 0.03）。对III组小鼠潜在性别效应的比较表明，胆汁螺杆菌感染的雌性小鼠比感染的雄性小鼠出现更严重的门静脉炎症（P < 0.01）。基于酶联免疫吸附测定（ELISA）的结果，III组11只6至8月龄的胆汁螺杆菌感染小鼠中有8只血清转化为胆汁螺杆菌外膜抗原。胆汁螺杆菌感染与SW小鼠的肝炎相关，并且可能混淆实验结果。
