Kowalski M L, Lewandowska A, Wozniak J, Makowska J, Jankowski A, DuBuske L
Department of Clinical Immunology and Allergy, Faculty of Medicine, Medical University, Lodz, Poland.
Allergy. 2005 Jan;60(1):80-5. doi: 10.1111/j.1398-9995.2005.00642.x.
Nasal polyp tissue which contains mast cells and eosinophils is similar to the inflamed airway mucosa in cellular composition and mediator content. This investigation assessed the effect of desloratadine (DL), on activation of cells in nasal polyp tissue. Polyps were obtained from 22 patients with chronic rhinosinusitis [nine aspirin acetylosalitic acid (ASA)-sensitive and 13 ASA-tolerant]. Polyp tissue was dispersed by digestion, and preincubated with DL and incubated with anti-immunoglobulin E (IgE) or calcium ionophore. LTC4, eosinophil cationic protein (ECP) and tryptase concentrations in supernatants were measured by immunoassays. Desloratadine (1, 10 and 50 microM) inhibited calcium ionophore-induced LTC4 release by a mean of 29%, 50% and 63% respectively, and anti-IgE-induced LTC4 release by a mean of 27%, 35% and 39% respectively. Calcium ionophore-induced tryptase release was inhibited 60% and 69% by 10 and 50 microM of DL, respectively, and anti-IgE-induced tryptase release was inhibited 33%, 47% and 66% for 1, 10 and 50 microM of DL. Desloratadine 10 microM and 50 microM inhibited ECP release by and 45% and 48% respectively. Polyp tissue from ASA-sensitive patients when compared with ASA-tolerant patients released at baseline significantly more ECP (medians 120.0 microg/ml, range: 69.0-182.0 vs 63.4 microg/ml, range: 3.7-172.0; P <0.05), but similar amounts of tryptase and LTC4. This study demonstrated that DL inhibits activation of both eosinophils and mast cells derived from a site of airway mucosal inflammation.
含有肥大细胞和嗜酸性粒细胞的鼻息肉组织在细胞组成和介质含量方面与炎症气道黏膜相似。本研究评估了地氯雷他定（DL）对鼻息肉组织中细胞活化的影响。从22例慢性鼻 - 鼻窦炎患者（9例对阿司匹林乙酰水杨酸[ASA]敏感，13例对ASA耐受）获取息肉。息肉组织经消化分散后，先用DL预孵育，再与抗免疫球蛋白E（IgE）或钙离子载体孵育。通过免疫测定法测量上清液中白三烯C4（LTC4）、嗜酸性粒细胞阳离子蛋白（ECP）和类胰蛋白酶的浓度。地氯雷他定（1、10和50微摩尔）分别平均抑制钙离子载体诱导的LTC4释放29%、50%和63%，以及抗IgE诱导的LTC4释放27%、35%和39%。10和50微摩尔的DL分别抑制钙离子载体诱导的类胰蛋白酶释放60%和69%，1、10和50微摩尔的DL分别抑制抗IgE诱导的类胰蛋白酶释放33%、47%和66%。10微摩尔和50微摩尔的地氯雷他定分别抑制ECP释放45%和48%。与ASA耐受患者相比，ASA敏感患者的息肉组织在基线时释放的ECP显著更多（中位数分别为120.0微克/毫升，范围：69.0 - 182.0与63.4微克/毫升，范围：3.7 - 172.0；P <0.05），但类胰蛋白酶和LTC4的释放量相似。本研究表明，DL抑制源自气道黏膜炎症部位的嗜酸性粒细胞和肥大细胞的活化。
