Baldacini O, Girardot R, Green G A, Rihn B, Monteil H
Institut de Bactériologie, Faculté de Médecine, Université Louis Pasteur, 3, Strasbourg, France.
Toxicon. 1992 Feb;30(2):129-40. doi: 10.1016/0041-0101(92)90466-i.
We compared the immunological properties and cytotoxic effects of Clostridium difficile toxin B and Clostridium sordellii toxin L. These two cytotoxins are immunologically related in that the cytotoxic effect of either toxin can be neutralized by the polyclonal antiserum prepared against either cytotoxin. On the other hand, polyclonal antiserum prepared against Clostridium difficile enterotoxin A did not cross-react with the cytotoxins B and L when tested by cytotoxic neutralization test nor by double immunodiffusion assay. However, despite this immunological relationship between toxins B and L, the morphological modifications observed in MacCoy cells induced by treatment with these cytotoxins are clearly distinct. We describe the first quantitative analysis of specific cellular parameters which illustrates the morphological differences induced by these cytotoxins. Moreover, immunocytochemical experiments show that, whereas disruption of microfilaments is observed with toxin B- and L-treatments, alterations of F-actin network are different in the cells treated with toxin B or L. The observation that the cellular modifications induced by toxin B- and toxin L-treatment differ suggests that the molecular mechanisms involved in the respective cytotoxicities are also likely to be different.
我们比较了艰难梭菌毒素B和索氏梭菌毒素L的免疫特性及细胞毒性作用。这两种细胞毒素在免疫方面存在关联，即针对任何一种细胞毒素制备的多克隆抗血清都能中和另一种毒素的细胞毒性作用。另一方面，当通过细胞毒性中和试验或双向免疫扩散试验检测时，针对艰难梭菌肠毒素A制备的多克隆抗血清与毒素B和L均无交叉反应。然而，尽管毒素B和L之间存在这种免疫关系，但用这些细胞毒素处理麦科伊细胞后观察到的形态学改变却明显不同。我们描述了对特定细胞参数的首次定量分析，该分析阐明了这些细胞毒素诱导的形态学差异。此外，免疫细胞化学实验表明，虽然毒素B和L处理均观察到微丝破坏，但毒素B或L处理的细胞中F-肌动蛋白网络的改变有所不同。毒素B和毒素L处理诱导的细胞改变不同这一观察结果表明，各自细胞毒性作用所涉及的分子机制也可能不同。
