祖细胞扩增：慢性肝炎中肝细胞再生的重要来源。
Progenitor cell expansion: an important source of hepatocyte regeneration in chronic hepatitis.
作者信息
Eleazar Jennifer A, Memeo Lorenzo, Jhang Jeffrey S, Mansukhani Mahesh M, Chin Steven, Park Soo Mi, Lefkowitch Jay H, Bhagat Govind
机构信息
Department of Pathology, College of Physicians and Surgeons, Columbia University, 630 West 168th Street, New York, NY 10032, USA.
出版信息
J Hepatol. 2004 Dec;41(6):983-91. doi: 10.1016/j.jhep.2004.08.017.
BACKGROUND/AIMS: Progenitor cell activation with subsequent maturation to hepatocytes and cells of the biliary lineage has been demonstrated in a variety of chronic liver diseases but the kinetics and magnitude of the progenitor cell response has not been adequately studied in detail in chronic hepatitis. We undertook this study to evaluate factors responsible for the progenitor cell/ductular response and further dissect the role of disease grade and stage as determinants of hepatocellular differentiation of bipotential progenitor cells in chronic hepatitis.
METHODS
Cytokeratin 7 (and 19) stained biopsies from patients with chronic hepatitis C (n = 47), hepatitis B (n = 20), and autoimmune hepatitis (n = 20) were studied. Ploidy analysis and proliferation indices were evaluated in a subset of cases.
RESULTS
Ductular reactions were present in the majority of cases (97%), appeared early in disease, and correlated with disease activity, while progenitor cell derived hepatocyes appeared later in disease and their extent correlated with disease stage. Proliferation indices of all cell types correlated with disease activity.
CONCLUSIONS
Progenitor cell derived hepatocytes accrue in chronic hepatitis, possibly related to native hepatocellular dysfunction. However, the fate of these hepatocytes is unclear.
背景/目的：在多种慢性肝病中已证实祖细胞被激活，随后成熟为肝细胞和胆管谱系细胞，但在慢性肝炎中，祖细胞反应的动力学和程度尚未得到充分详细研究。我们开展这项研究以评估导致祖细胞/小胆管反应的因素，并进一步剖析疾病分级和分期作为慢性肝炎中双能祖细胞肝细胞分化决定因素的作用。
方法
研究了来自丙型肝炎（n = 47）、乙型肝炎（n = 20）和自身免疫性肝炎（n = 20）患者的细胞角蛋白7（和19）染色活检标本。在部分病例中评估了倍体分析和增殖指数。
结果
大多数病例（97%）存在小胆管反应，在疾病早期出现，并与疾病活动相关，而祖细胞衍生的肝细胞在疾病后期出现，其范围与疾病分期相关。所有细胞类型的增殖指数均与疾病活动相关。
结论
慢性肝炎中存在祖细胞衍生的肝细胞，可能与天然肝细胞功能障碍有关。然而，这些肝细胞的命运尚不清楚。
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