Liu Yuan, Jin Yan, Wang Xinwen
Department of Oral Histology & Pathology, Stomatological College, Fourth Military Medical University, Xi'an Shaanxi, PR China.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2004 Nov;18(6):502-4.
To construct a tissue engineering skin containing capillary-like network by employing tissue engineering method.
The numan umbilical vein endothelial cells(HUVECs) were isolated from a new-born umbilical cord. The keratinocytes and dermal fibroblasts were isolated from a new-born foreskin biopsy. After the collagen gel was prepared, the fibroblasts and the vascular endothelial cells were added in a ratio of 1 to 1 to construct a skin substitute containing capillary-like network. The skin substitute was observed by HE staining and immuno histochemical staining (VIII factor). The reconstructed skin containing capillary-like network was used to repair the nude mice skin defects in the experimental group. The tissue engineering skin containing no vascular endothelial cells was used in control group.
Capillary-like network could be observed in the dermal layer of the tissue engineering skin, and the nude mice skin defects were repaired by the skin substitutes in the experimental group. In control group, no capillary-like network was found.
The tissue engineering skin containing capillary-like network is successfully constructed in vitro and can be used to repair the full-thickness skin defects.
采用组织工程方法构建含毛细血管样网络的组织工程皮肤。
从新生儿脐带中分离人脐静脉内皮细胞（HUVECs）。从新生儿包皮活检组织中分离角质形成细胞和真皮成纤维细胞。制备胶原凝胶后，按1:1的比例加入成纤维细胞和血管内皮细胞，构建含毛细血管样网络的皮肤替代物。通过苏木精-伊红（HE）染色和免疫组织化学染色（VIII因子）观察皮肤替代物。实验组用含毛细血管样网络的重构皮肤修复裸鼠皮肤缺损。对照组用不含血管内皮细胞的组织工程皮肤。
在组织工程皮肤的真皮层可观察到毛细血管样网络，实验组的皮肤替代物修复了裸鼠皮肤缺损。对照组未发现毛细血管样网络。
成功在体外构建了含毛细血管样网络的组织工程皮肤，可用于修复全层皮肤缺损。
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