Wang Hong-Tao, Chen Bi, Tang Chao-Wu, Hu Da-Hai
Department of Burn Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, PR China.
Zhonghua Shao Shang Za Zhi. 2007 Jun;23(3):222-4.
To investigate the feasibility of fabricating tissue engineering skin with human hair follicle bulge cells (HFBCs) to repair full-thickness skin wound.
HFBCs and dermal papilla cells (DPCs) isolated from human fetal hair follicles by collagenase digestion were cultured, purified and passaged. PGA-collagen scaffolds as bioengineered dermis were randomly divided into A and B groups. The HFBCs and DPCs (1 : 2) were seeded in scaffolds of group A and the equal amount of DPCs was seeded in scaffolds of group B as control. Then the keratinocyte sheets were seeded onto the surfaces of the scaffolds as bioengineered epidermis. The tissue engineering skins were then transplanted to repair the full-thickness wound on the back of nude mice. The wound healing process was observed and the plant histological changes of the transplanted engineered skin was observed with light microscope on 2, 4, 6 post-operation weeks (POW).
The full-thickness defect of nude mice in A and B groups could be effectively repaired by bioengineered skins. On 2 POW, integral epidermal and dermal structures were observed in the wounds in A and B groups, with thin epithelial layer and basement membrane. On 4 POW, epithelial layer became thickening and rete pegs formation was observed in basement membrane in A group, but only thickening of epithelial layer was observed in B group. On 6 POW, rete pegs structure was seen to descend and hair-follicle-like structure was formed, while only thickened epithelial layer with flat basement membrane were formed in B group.
From the composite skin engineered with PGA-collagen hybrid scaffolds and keratinocytes, HFBCs and DPCs could effectively repair the full-thickness skin defect of nude mice. The hair follicle stem cells participate in the process of anatomic repair of wound, and might be able to induce the repair of skin structure and function.
探讨用人毛囊隆突细胞（HFBCs）构建组织工程皮肤修复全层皮肤创面的可行性。
采用胶原酶消化法从人胎儿毛囊中分离出HFBCs和真皮乳头细胞（DPCs），进行培养、纯化及传代。将聚乙醇酸-胶原支架作为生物工程真皮，随机分为A、B两组。将HFBCs与DPCs按1∶2接种于A组支架，B组支架接种等量DPCs作为对照。然后将角质形成细胞片接种于支架表面作为生物工程表皮。将组织工程皮肤移植到裸鼠背部修复全层创面。观察创面愈合过程，并于术后2、4、6周用光镜观察移植工程皮肤的组织学变化。
A、B组生物工程皮肤均可有效修复裸鼠全层皮肤缺损。术后2周，A、B组创面可见完整的表皮和真皮结构，上皮层薄，有基底膜。术后4周，A组上皮层增厚，基底膜有 rete 嵴形成，而B组仅上皮层增厚。术后6周，A组 rete 嵴结构下移，形成毛囊样结构，而B组仅形成增厚的上皮层和平坦的基底膜。
由聚乙醇酸-胶原混合支架、角质形成细胞、HFBCs和DPCs构建的复合皮肤可有效修复裸鼠全层皮肤缺损。毛囊干细胞参与创面的解剖修复过程，可能诱导皮肤结构和功能的修复。
