Souverain Sandrine, Rudaz Serge, Veuthey Jean-Luc
Laboratory of Pharmaceutical and Analytical Chemistry, School of Pharmacy, University of Geneva, 1211 Geneva 4, Switzerland.
J Chromatogr A. 2004 Nov 26;1058(1-2):61-6.
Matrix effect on mass spectrometry response was investigated with commercially available electrospray ionisation (ESI) and atmospheric pressure chemical ionisation (APCI) sources coupled with a single quadruple mass spectrometer. A post-column infusion system was used to observe the MS signal alterations of methadone, selected as the model compound, in plasma. For this purpose, two sample preparation procedures were tested: (1) conventional off-line sample preparations with liquid-liquid extraction (LLE), solid-phase extraction (SPE) and protein precipitation (PP) with perchloric acid (PA) and acetonitrile (ACN) and (2) on-line SPE with two different extraction columns packed with a large particle support (LPS) and with restricted access material (RAM), respectively. Whatever the sample preparation procedures, APCI source appeared to be less liable to matrix effect than ESI source. Among the different off-line sample preparations, LLE was the most efficient extraction procedure. With other techniques, MS signal was affected not only by endogenous material but also by procedure introduced compounds. Moreover, on-line SPE-LC-MS configuration exhibited matrix effects, which depend on the API source and the extraction support.
采用市售电喷雾电离（ESI）和大气压化学电离（APCI）源与单四极杆质谱仪联用，研究了基质对质谱响应的影响。采用柱后注入系统观察血浆中作为模型化合物的美沙酮的质谱信号变化。为此，测试了两种样品制备方法：（1）采用液液萃取（LLE）、固相萃取（SPE）以及用高氯酸（PA）和乙腈（ACN）进行蛋白质沉淀（PP）的传统离线样品制备方法，以及（2）分别使用填充大颗粒载体（LPS）和限进材料（RAM）的两种不同萃取柱的在线SPE方法。无论采用何种样品制备方法，APCI源似乎比ESI源更不易受到基质效应的影响。在不同的离线样品制备方法中，LLE是最有效的萃取方法。采用其他技术时，质谱信号不仅受到内源性物质的影响，还受到方法引入的化合物的影响。此外，在线SPE-LC-MS配置表现出基质效应，这取决于API源和萃取载体。
