[通过双色荧光原位杂交对少精子症的46, XY/47, XXY患者精子中性染色体的分离]
[Segregation of sex chromosomes in the spermatozoa of 46, XY/47, XXY patients with oligozoospermia by dual fluorescence in-situ hybridization].
作者信息
Cui Yingxia, Ge Yifeng, Wang Yunhua, Zhou Qin, Wang Yongmei, Zhu Peiyuan, Shang Xuejun, Huang Yufeng
机构信息
Laboratory of Reproduction & Genetics, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China.
出版信息
Zhonghua Nan Ke Xue. 2004 Nov;10(11):841-3.
OBJECTIVE
To observe the segregation of sex chromosomes in the spermatozoa of a 46, XY/47, XXY patient with oligozoospermia.
METHODS
The number of X and Y chromosomes of the ejaculated spermatozoa from the patient with mosaic 46, XY/47, XXY was analysed by X/Y dual fluorescence in-situ hybridization (FISH).
RESULTS
Of the 100 spermatozoa analysed, 97 showed either one X chromosome-specific green signal or one Y-chromosome-specific red Y signal in each spermatozoon and only 3 showed no signal. The frequencies of X- and Y-bearing spermatozoa were 49% and 48% respectively. The ratio of X- to Y-bearing spermatozoa was about 1:1 as expected. There was no statistical difference between the chromosome XX and XY frequencies in each spermatozoon from the patient in comparison with those estimated in the control.
CONCLUSION
The spermatozoa of 46, XX/47, XXY mosaic patients have a normal gonosomal complement, which allows infertility treatment to be carried out by ICSI.
目的
观察一名患有少精子症的46,XY/47,XXY患者精子中性染色体的分离情况。
方法
采用X/Y双色荧光原位杂交（FISH）技术分析46,XY/47,XXY嵌合体患者射出精子中的X和Y染色体数量。
结果
在分析的100个精子中，97个精子每个都显示出一个X染色体特异性绿色信号或一个Y染色体特异性红色信号，只有3个精子无信号。含X精子和含Y精子的频率分别为49%和48%。含X精子与含Y精子的比例约为1:1，符合预期。与对照组估计值相比，该患者每个精子中XX和XY染色体频率无统计学差异。
结论
46,XX/47,XXY嵌合体患者的精子具有正常的性染色体组成，这使得可以通过卵胞浆内单精子注射（ICSI）进行不育治疗。
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