Nagvenkar Punam, Zaveri Kusum, Hinduja Indira
Jaslok Hospital and Research Centre, Mumbai, India.
Fertil Steril. 2005 Oct;84(4):925-31. doi: 10.1016/j.fertnstert.2005.04.048.
To determine the incidence of disomy and diploidy for chromosomes 18, X, and Y in the sperm samples of severe oligozoospermic (<5 x 10(6) spermatozoa/mL) and oligozoospermic (5-20 x 10(6) spermatozoa/mL) men undergoing intracytoplasmic sperm injection (ICSI) and to evaluate the influence of sperm aneuploidy on pregnancy outcome.
Prospective study.
Infertility clinic and genetic laboratory.
PATIENT(S): Fifteen patients with severe oligozoospermia, 15 patients with oligozoospermia, and 10 normal fertile donors.
INTERVENTION(S): Fluorescence in-situ hybridization (FISH) performed on sperm samples.
MAIN OUTCOME MEASURE(S): The frequency of disomy and diploidy for chromosomes 18, X, and Y was analyzed using FISH, and the clinical outcome after ICSI was correlated.
RESULT(S): Significantly greater frequencies of XY, YY disomy and diploidy were observed in severe oligozoospermic men compared with oligozoospermic and normozoospermic men. Although the fertilization rate was similar, the pregnancy rate was higher in the group with oligozoospermia versus severe oligozoospermia.
CONCLUSION(S): This study demonstrated the presence of an elevated sperm aneuploidy rate in patients with low semen quality. Additionally, the data show a negative influence of sperm chromosome abnormalities on ICSI outcome.
确定重度少精子症（精子浓度<5×10⁶/mL）和少精子症（精子浓度5 - 20×10⁶/mL）男性接受卵胞浆内单精子注射（ICSI）时，其精子样本中18号、X和Y染色体的二体性和二倍体发生率，并评估精子非整倍体对妊娠结局的影响。
前瞻性研究。
不孕不育诊所和基因实验室。
15例重度少精子症患者、15例少精子症患者和10名正常生育男性供者。
对精子样本进行荧光原位杂交（FISH）。
使用FISH分析18号、X和Y染色体的二体性和二倍体频率，并将ICSI后的临床结局与之相关联。
与少精子症和正常精子症男性相比，重度少精子症男性中XY、YY二体性和二倍体的频率显著更高。尽管受精率相似，但少精子症组的妊娠率高于重度少精子症组。
本研究表明精液质量低的患者精子非整倍体率升高。此外，数据显示精子染色体异常对ICSI结局有负面影响。
