Giavedoni Luis D, Schlabritz-Loutsevitch Natalia, Hodara Vida L, Parodi Laura M, Hubbard Gene B, Dudley Donald J, McDonald Thomas J, Nathanielsz Peter W
Southwest National Primate Research Center, Southwest Foundation for Biomedical Research, San Antonio, TX, USA.
J Reprod Immunol. 2004 Dec;64(1-2):121-32. doi: 10.1016/j.jri.2004.09.001.
Baboons are very similar to humans in ontogeny, reproductive physiology, and placentation, and thus serve as an excellent nonhuman primate model for use in both normative and perturbation studies of pregnancy that cannot be performed on pregnant women. However, little is known about the changes induced by normal pregnancy in the maternal and fetal baboon in lymphocyte subset composition, and lymphocyte activation and proliferation. We performed multicolor flow cytometry analysis of peripheral venous blood samples obtained from pregnant baboons at mid-gestation (0.5 G), and from matched fetal heart (FH) and umbilical cord (UC) blood at the end of gestation (0.95 G). Compared with their mothers at 0.95 G, fetal lymphocytes had higher percentages of B and CD4+ T cells, and lower numbers of NK and CD8+ T cells. When comparing pregnant baboons at 0.5 and 0.95 G, we also found that pregnancy induces immune stimulation, measured as higher activation without proliferation of CD8+ T and NK cells in the maternal circulation. Our study adds new data to support the notion of pregnancy-induced immune activation and strengthens the value of the baboon as a nonhuman primate model for studies pertinent to human reproductive physiology, pathology, and vaccination.
狒狒在个体发育、生殖生理学和胎盘形成方面与人类非常相似，因此是一种出色的非人类灵长类动物模型，可用于对孕妇无法进行的妊娠规范研究和干扰研究。然而，关于正常妊娠对狒狒母体和胎儿淋巴细胞亚群组成、淋巴细胞激活和增殖的影响，我们知之甚少。我们对妊娠中期（0.5G）的怀孕狒狒以及妊娠末期（0.95G）匹配的胎儿心脏（FH）和脐带（UC）血样进行了外周静脉血样本的多色流式细胞术分析。与0.95G时的母亲相比，胎儿淋巴细胞中B细胞和CD4+T细胞的百分比更高，NK细胞和CD8+T细胞的数量更低。在比较0.5G和0.95G的怀孕狒狒时，我们还发现妊娠会诱导免疫刺激，表现为母体循环中CD8+T细胞和NK细胞激活增加但无增殖。我们的研究增加了新的数据来支持妊娠诱导免疫激活的观点，并强化了狒狒作为与人类生殖生理学、病理学和疫苗接种相关研究的非人类灵长类动物模型的价值。
