Bukowska Dorota, Mierzejewska-Krzyzowska Barbara, Zguczyński Leszek
Department of Neurobiology, University School of Physical Education, 55 Grunwaldzka St., 60-352 Poznań, Poland.
Neurosci Res. 2005 Jan;51(1):15-24. doi: 10.1016/j.neures.2004.09.011.
Projections of the nucleus reticularis tegmenti pontis (NRTP) to the cerebellar paramedian lobule were examined in the rabbit by means of the double fluorescent retrograde tract-tracing method. The rabbit NRTP is composed of a medial, large part comprising zones A (dorsomedial), B (central) and C (lateral), and of a lateral, small part (the processus tegmentosus lateralis; PTL). Following unilateral injections of Fast Blue (FB) into the rostral part of the paramedian lobule (rPML) and of Diamidino Yellow (DY) into the caudal part (cPML), known to receive spinal inputs from forelimb and hindlimb, respectively, substantial numbers of single labeled neurons were found in all bilateral NRTP divisions, apart from the zone C. Most projection neurons to the PML were located in the medial and medioventral regions of the zone B. Smaller numbers of projection neurons were located in the PTL, zone A and outside the zone B among fibers of the medial lemniscus. The pattern of FB and DY labeling suggested that neurons projecting to the rPML and cPML originated in common rather than separate regions within the NRTP. In addition, a small percentage (mean 1.3%) of double FB+DY labeled neurons were detected with a clear contralateral preponderance, among single labeled FB or DY cells. In spite of the rarity, all the NRTP neurons giving rise to intralobular collateral projections can be regarded as potential sources of simultaneous modulating influences upon two functional different forelimb (rPML) and hindlimb (cPML) regions. The findings have been discussed in relation to earlier studies in other species and commented on with respect to the possible functional meaning of these projections.
采用双荧光逆行束路追踪法，在兔中研究了脑桥被盖网状核（NRTP）向小脑旁正中小叶的投射。兔NRTP由内侧的大部分和外侧的小部分组成，内侧大部分包括A区（背内侧）、B区（中央）和C区（外侧），外侧小部分为外侧被盖突（PTL）。分别向已知分别接受来自前肢和后肢脊髓输入的旁正中小叶头端部分（rPML）注射快蓝（FB），向尾端部分（cPML）注射二脒基黄（DY）后，除C区外，在所有双侧NRTP分区中均发现了大量单标记神经元。投射到PML的大多数神经元位于B区的内侧和内侧腹侧区域。较少数量的投射神经元位于PTL、A区以及内侧丘系纤维中的B区外侧。FB和DY标记模式表明，投射到rPML和cPML的神经元起源于NRTP内的共同区域而非分开的区域。此外，在单标记的FB或DY细胞中，检测到一小部分（平均1.3%）双FB+DY标记神经元，且对侧明显占优势。尽管这种情况很少见，但所有产生小叶内侧支投射的NRTP神经元都可被视为对两个功能不同的前肢（rPML）和后肢（cPML）区域同时产生调节影响的潜在来源。已结合其他物种的早期研究对这些发现进行了讨论，并就这些投射可能的功能意义进行了评论。
