Haim Hillel, Steiner Israel, Panet Amos
Department of Virology, Hadassah Medical School, The Hebrew University, Jerusalem, Israel.
J Virol. 2005 Jan;79(1):622-5. doi: 10.1128/JVI.79.1.622-625.2005.
To override the diffusion-limited adsorption step of viral infection, we magnetically synchronized cell attachment. Human immunodeficiency virus type 1-based lentivirus preparations were rendered magnetically reactive by association with magnetite nanoparticles, 50 nm in diameter. Application of a magnetic field resulted in immediate redistribution of the viral inoculum to the cell-associated state and completion of the productive adsorption process within 1 min. Independent of adsorption time, viral concentration, and diffusion rate, infection subsequently progressed by the receptor-mediated entry mechanism. Synchronization of this rate-limiting step of infection may now be applied to analyze isolated events in the viral replication sequence.
为了克服病毒感染中扩散限制的吸附步骤,我们对细胞附着进行了磁同步。通过与直径为50纳米的磁铁矿纳米颗粒结合,使基于1型人类免疫缺陷病毒的慢病毒制剂具有磁反应性。施加磁场导致病毒接种物立即重新分布到细胞相关状态,并在1分钟内完成有效吸附过程。感染随后通过受体介导的进入机制进行,与吸附时间、病毒浓度和扩散速率无关。现在,感染这一限速步骤的同步可用于分析病毒复制序列中的孤立事件。