Lu Hui-Juan, Gong Zu-Xun
Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2004 Apr;30(2):121-6.
Currently used plant transformation systems require selectable marker genes encoding antibiotic or herbicide resistance, along with the gene of interest, to select transformed cells from a large population of mostly untransformed cells. The continued presence of these selectable markers, especially in food crop, is of increasing public concern. The generation of selectable marker-free transgenic plant is one of the new projects in plant biotechnology research. Two techniques, segregation excision and recombination excision, for removal of selectable marker genes are described in this article. The advances in producing selectable marker-free transgenic plants are reviewed too.
目前使用的植物转化系统需要编码抗生素或除草剂抗性的选择标记基因以及目的基因，以便从大量未转化细胞中筛选出转化细胞。这些选择标记的持续存在，尤其是在粮食作物中，越来越引起公众的关注。培育无选择标记转基因植物是植物生物技术研究的新项目之一。本文描述了两种去除选择标记基因的技术，即分离切除和重组切除。同时也综述了培育无选择标记转基因植物方面的进展。
