NG-硝基-L-精氨酸甲酯对氨茶碱作用于大鼠膈肌的效应增强：细胞外钙的作用

NG-nitro-L-arginine methyl ester-induced potentiaton of the effect of aminophylline on rat diaphragm: the role of extracellular calcium.

作者信息

Stojanovic Radan, Todorović Zoran, Nesić Zorica, Vućković Sonja, Cerovac-Cosić Natasa, Prostran Milica

机构信息

Department of Clinical Pharmacology, Pharmacology and Toxicology, School of Medicine University of Belgrade, Belgrade, Serbia and Montenegro.

出版信息

J Pharmacol Sci. 2004 Dec;96(4):493-8. doi: 10.1254/jphs.sce04001x. Epub 2004 Dec 15.

DOI:10.1254/jphs.sce04001x

PMID:15599090

Abstract

The role of extracellular calcium in the interaction between intracellular cAMP and nitric oxide (NO)/cGMP on the contractility of rat diaphragm pretreated with cumulative concentrations of aminophylline (0.36 - 3.60 mM) was investigated. In a Ca2+-free medium, NG-nitro-L-arginine methyl ester (L-NAME) (1 and 3 mM) depressed tension developed (Td) and also aminophylline-induced potentiation of Td in a concentration-dependent manner. Verapamil (2.5 microM) or nicardipine (20 microM) significantly antagonized the potentiating effect of L-NAME on Td in a calcium-containing medium. However, in the presence of verapamil or nicardipine, L-NAME still produced statistically significant potentiation of the cumulative concentrations of aminophylline (0.36 - 3.60 mM), given in the second series.

摘要

研究了细胞外钙在细胞内cAMP与一氧化氮（NO）/环磷酸鸟苷（cGMP）相互作用中对用累积浓度氨茶碱（0.36 - 3.60 mM）预处理的大鼠膈肌收缩性的作用。在无钙培养基中，Nω-硝基-L-精氨酸甲酯（L-NAME）（1和3 mM）以浓度依赖性方式降低了张力发展（Td）以及氨茶碱诱导的Td增强作用。维拉帕米（2.5 μM）或尼卡地平（20 μM）在含钙培养基中显著拮抗L-NAME对Td的增强作用。然而，在存在维拉帕米或尼卡地平的情况下，L-NAME对第二组中给出的累积浓度氨茶碱（0.36 - 3.60 mM）仍产生统计学上显著的增强作用。