Demers Caroline N, Antoniou John, Mwale Fackson
Division of Orthopaedic Surgery, Lady Davis Institute for Medical Research, SMBD-Jewish General Hospital, McGill University, Montreal, Quebec, Canada.
Spine (Phila Pa 1976). 2004 Dec 15;29(24):2793-9. doi: 10.1097/01.brs.0000147744.74215.b0.
The contents of DNA, proteoglycan, type II collagen, and denatured type II collagen in the bovine coccygeal intervertebral discs were examined in situ in relation to disc level, age, and tissue region.
To determine whether bovine coccygeal discs are a suitable model to study human lumbar discs.
Bovine coccygeal discs have been suggested as a suitable alternative model because they are readily available, in contrast to human discs, and represent a common source of tissue in the disc field. However, it is not known whether the changes in matrix contents in bovine coccygeal discs are similar to those found in the human lumbar spine.
Intervertebral discs from bovine tails were dissected into the nucleus pulposus (NP) and anulus fibrosus (AF). Tissues were weighed and analyzed for matrix contents using specific assays.
Similar to water content, the proteoglycan content was higher in the NP than in the AF. Water content of the bovine NP did not change with age, unlike the proteoglycan content, which decreased. type II collagen content was higher in the NP than in the AF, and both did not change overall significantly with age. The percent of denatured type II collagen decreased with age only in the NP. The DNA content did not vary with age in the AF and in the NP.
Differences in matrix contents exist between the bovine coccygeal discs and the human lumbar spine. Thus, caution must be exercised when using the bovine tail as a model for the human lumbar spine in biochemical studies.
对牛尾椎椎间盘内的DNA、蛋白聚糖、II型胶原蛋白和变性II型胶原蛋白含量进行原位检测，分析其与椎间盘节段、年龄和组织区域的关系。
确定牛尾椎椎间盘是否是研究人类腰椎间盘的合适模型。
牛尾椎椎间盘被认为是一种合适的替代模型，因为与人类椎间盘不同，它们很容易获得，是椎间盘领域常见的组织来源。然而，尚不清楚牛尾椎椎间盘基质含量的变化是否与人类腰椎的变化相似。
将牛尾的椎间盘解剖为髓核（NP）和纤维环（AF）。对组织进行称重，并使用特定检测方法分析基质含量。
与水分含量相似，蛋白聚糖含量在髓核中高于纤维环。与蛋白聚糖含量下降不同，牛髓核的水分含量不随年龄变化。II型胶原蛋白含量在髓核中高于纤维环，且两者总体上均不随年龄显著变化。仅在髓核中，变性II型胶原蛋白的百分比随年龄下降。纤维环和髓核中的DNA含量不随年龄变化。
牛尾椎椎间盘与人类腰椎在基质含量上存在差异。因此，在生物化学研究中使用牛尾作为人类腰椎的模型时必须谨慎。
