Mueller Nicolas J, Kuwaki Kenji, Dor Frank J M F, Knosalla Christoph, Gollackner Bernd, Wilkinson Robert A, Sachs David H, Cooper David K C, Fishman Jay A
Infectious Diseases Division, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, GRJ 504, Boston, MA 02114, USA.
Transplantation. 2004 Nov 27;78(10):1449-53. doi: 10.1097/01.tp.0000141361.68446.1f.
Xenotransplantation using pigs as the source species for organs carries a potential risk for transmission and activation of porcine herpesviruses. Activation of porcine cytomegalovirus (PCMV) in pig-to-baboon xenotransplantation is associated with xenograft injury and possibly an increased incidence of consumptive coagulopathy (CC).
To further investigate the role of PCMV activation in the occurrence of CC, a strategy to exclude PCMV from the donor was developed. To exclude PCMV, piglets were early-weaned and raised separated from other swine. These piglets were used as donors in an experimental protocol of pig-to-baboon heart xenotransplantation.
Early weaning of piglets was successful in excluding PCMV. Use of PCMV-free cardiac porcine xenografts in baboons resulted in prolonged graft survival and prevented consumptive coagulopathy in all recipients.
The use of PCMV-free cardiac grafts is beneficial in reducing the direct effects of PCMV activation in the graft (tissue damage) and the indirect effects of PCMV activation in the recipient (consumptive coagulopathy).
使用猪作为器官来源物种进行异种移植存在猪疱疹病毒传播和激活的潜在风险。猪巨细胞病毒（PCMV）在猪到狒狒的异种移植中的激活与异种移植物损伤相关，并且可能与消耗性凝血病（CC）的发病率增加有关。
为了进一步研究PCMV激活在CC发生中的作用，制定了一种从供体中排除PCMV的策略。为了排除PCMV，仔猪提前断奶并与其他猪分开饲养。这些仔猪被用作猪到狒狒心脏异种移植实验方案中的供体。
仔猪早期断奶成功排除了PCMV。在狒狒中使用无PCMV的猪心脏异种移植物可延长移植物存活时间，并防止所有受体发生消耗性凝血病。
使用无PCMV的心脏移植物有利于减少PCMV激活在移植物中的直接影响（组织损伤）以及PCMV激活在受体中的间接影响（消耗性凝血病）。
