[背根神经节卫星细胞中神经生长因子、脑源性神经营养因子和神经营养素-3的表达]
[Expression of NGF, BDNF and NT-3 in satellite cells of dorsal root ganglion].
作者信息
Liu Fen, Wang Tinghua, Li Ming, Wu Linyan, Feng Zhongtang, Wang Tewei
机构信息
Institute of Neuroscience, Kunming Medical College, Kunming 650031, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Jan;34(1):36-9.
OBJECTIVE
To investigate the distribution of NGF, BDNF, NT-3 and their mRNA in satellite cells of dorsal root ganglion (DRG) in adult cat.
METHODS
Five male adult cats were used and their L6 DRGs unilateral side were taken and sectioned into frozen sections 20 microm in thickness respectively. The sections were stained by immunohistochemistry ABC method (NGF antibody 1:1000, Sigma; BDNF antibody 1:500, Santa Cruz; NT-3 antibody 1:1500, Santa Cruz), and in situ hybridization study(cRNA probe 1:100).
RESULTS
NGF, BDNF, NT-3 and their mRNA were observed in some satellite cells. The positive signal of mRNA for NGF, BDNF and NT-3 was observed in cytoplasm. However, there was difference in the subcellular localization of protein for the three factors. BDNF immunoreactivity (BDNF-IR) was located in cytoplasm, while NGF-IR and NT-3-IR were located in nucleus.
CONCLUSION
The results demonstrated that some satellite cells contain NGF, BDNF and NT-3 as well as their mRNA. The difference of subcellular localization for NGF, BDNF and NT-3 indicated that the functional mechanism of each factor in satellite cells in adult cats may be different.
目的
研究成年猫背根神经节（DRG）卫星细胞中神经生长因子（NGF）、脑源性神经营养因子（BDNF）、神经营养因子-3（NT-3）及其mRNA的分布。
方法
选用5只成年雄性猫，取其一侧L6背根神经节，分别制成20微米厚的冰冻切片。切片采用免疫组织化学ABC法染色（NGF抗体1:1000，Sigma公司；BDNF抗体1:500，Santa Cruz公司；NT-3抗体1:1500，Santa Cruz公司），并进行原位杂交研究（cRNA探针1:100）。
结果
在部分卫星细胞中观察到NGF、BDNF、NT-3及其mRNA。在细胞质中观察到NGF、BDNF和NT-3的mRNA阳性信号。然而，这三种因子蛋白的亚细胞定位存在差异。BDNF免疫反应性（BDNF-IR）位于细胞质中，而NGF-IR和NT-3-IR位于细胞核中。
结论
结果表明，部分卫星细胞含有NGF、BDNF和NT-3及其mRNA。NGF、BDNF和NT-3亚细胞定位的差异表明，成年猫卫星细胞中各因子的功能机制可能不同。
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