Dai Ping, Xiyang Yan-Bin
Institute of Neuroscience, Kunming Medical College, Kunming 650031, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 Jul;40(4):676-8.
To study the expression of NGF, BDNF and NT-3 in spinal cord of growing embryonic chicken.
The lumbar segments of the spinal cords were obtained from chick embryos at 7 day (E7) and 14 day (E14) respectively. The distribution of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) was examined in frozen sections by immunohistochemistry, and their level was determined by Western blot.
At E7, intensive NGF was detected mainly in the white matter of ventralateral zone, and BDNF was seen in both perikaryon and neurite of neurons, in the gray matter of the ventral horn, while NT-3 existed mainly within parts of the ventrolateral region of the spinal cord. Comparatively, at E14, NGF increased apparently in both the white and the gray matter. And BDNF presents in neuronal cell bodies and some axon of the dorsal horn, while NT-3 positive neurons were detected in the intermediate zone and the dorsal horn. Western-blot analysis confirmed the level of NGF, BDNF and NT-3 were all up regulated from E7 to E14 in the spinal cord of embryonic chicken during development.
The increase of NGF, BDNF and NT-3 in the dorsal horn was concomitant with the development of spinal cord from E7 to E14, suggesting that NGF, BDNF and NT-3 may be associated with the growth of the spinal cord during development.
研究神经生长因子（NGF）、脑源性神经营养因子（BDNF）和神经营养素-3（NT-3）在生长发育中的鸡胚脊髓中的表达。
分别从7日龄（E7）和14日龄（E14）的鸡胚获取腰段脊髓。采用免疫组织化学方法检测冰冻切片中神经生长因子（NGF）、脑源性神经营养因子（BDNF）和神经营养素-3（NT-3）的分布，并用蛋白质免疫印迹法测定其水平。
在E7时，密集的NGF主要在腹外侧区白质中检测到，BDNF可见于神经元的胞体和神经突以及腹角灰质中，而NT-3主要存在于脊髓腹外侧部分区域。相比之下，在E14时，NGF在白质和灰质中均明显增加。BDNF存在于背角的神经元细胞体和一些轴突中，而在中间带和背角检测到NT-3阳性神经元。蛋白质免疫印迹分析证实，在胚胎鸡脊髓发育过程中，从E7到E14，NGF、BDNF和NT-3的水平均上调。
从E7到E14，背角中NGF、BDNF和NT-3的增加与脊髓的发育同步，提示NGF、BDNF和NT-3可能与发育过程中脊髓的生长有关。
