[高密度脂蛋白对氧化型低密度脂蛋白诱导的内皮细胞凋亡的影响]
[Impact of high-density lipoprotein on the apoptosis of endothelial cells induced by oxidized low-density lipoprotein].
作者信息
Zhang Qing, Zeng Zhi, Ren Min, Chen Yucheng, Huang Minghui
机构信息
Department of Cardiology, West China Hospital, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2003 Jan;34(1):67-9.
OBJECTIVE
To investigate the impact of HDL on oxLDL-induced apoptosis of endothelial cells and relevant mechanisms.
METHODS
Human umbilical venous endothelial cells (ECV-304) were exposed to oxLDL or oxLDL + HDL. The apoptosis of the endothelial cells was checked by the Flow cytometry and DNA electrophoresis, while caspase-3 activity was meaured simultaneously.
RESULTS
The apoptosis rates induced by 100 microg/ml and 200 microg/ml of oxLDL were 28.40 + 5.30% and 34.7 +/- 4.64%, with caspase-3 activity of 166. 01 +/- 16.44 and 263.74 +/- 46.62 pmol/(min x mg pro), respectively. The DNA electrophoresis showed typical ladders. With exposure to HDL (200 mixrog/ml), the above apoptosis rates dropped to 8.06 +/- 2.35% and 9.40 +/- 2.58% respectively. There were no apoptotic ladders while the caspase-3 activities were significantly reduced (67.73 +/- 14.83 and 111.26 +/- 27.13 pmol/(min x mg pro).
CONCLUSIONS
HDL can significantly reduce the apoptosis of cultured endothelial cells induced by oxLDL. This effect may be mediated via suppression of caspase-3 activity.
目的
探讨高密度脂蛋白（HDL）对氧化型低密度脂蛋白（oxLDL）诱导的内皮细胞凋亡的影响及其相关机制。
方法
将人脐静脉内皮细胞（ECV-304）暴露于oxLDL或oxLDL + HDL中。通过流式细胞术和DNA电泳检测内皮细胞的凋亡情况，同时测定半胱天冬酶-3（caspase-3）活性。
结果
100μg/ml和200μg/ml的oxLDL诱导的凋亡率分别为28.40 + 5.30%和34.7 +/- 4.64%，caspase-3活性分别为166.01 +/- 16.44和263.74 +/- 46.62 pmol/（min x mg蛋白）。DNA电泳显示典型的梯状条带。当加入HDL（200μg/ml）后，上述凋亡率分别降至8.06 +/- 2.35%和9.40 +/- 2.58%。此时无凋亡梯状条带，且caspase-3活性显著降低（67.73 +/- 14.83和111.26 +/- 27.13 pmol/（min x mg蛋白）。
结论
HDL可显著降低oxLDL诱导的培养内皮细胞凋亡。这种作用可能是通过抑制caspase-3活性介导的。
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