Chianese L, Mauriello R, Moio L, Intorcia N, Addeo F
Istituto di Industrie Agrarie, Università di Napoli Federico II, Portici, Italia.
J Dairy Res. 1992 Feb;59(1):39-47. doi: 10.1017/s0022029900030223.
Discontinuous PAGE at alkaline and acid pH, polyacrylamide gel isoelectric focusing, two dimensional electrophoresis and immunoblotting have been used to study the heterogeneity of sheep caseins. Three main phenotypes were selected either because of mobility at alkaline and acid pH of the individual alpha s components or because of their relative intensity. On electrophoresis at alkaline pH, one phenotype showed two distinct bands of lower electrophoretic mobility than beta 1- and beta 2-caseins. A detailed study of these components using immunospecific detection with beta-casein antiserum showed that these minor components of ovine casein may have a polypeptide chain similar to that of beta 1- and beta 2-caseins. Complete electrophoretic patterns of the casein components in some individual milks are also presented.
采用碱性和酸性pH条件下的不连续聚丙烯酰胺凝胶电泳、聚丙烯酰胺凝胶等电聚焦、二维电泳及免疫印迹法研究了绵羊酪蛋白的异质性。根据单个αs组分在碱性和酸性pH条件下的迁移率或其相对强度，选择了三种主要表型。在碱性pH条件下进行电泳时，一种表型显示出两条电泳迁移率低于β1-和β2-酪蛋白的明显条带。使用β-酪蛋白抗血清进行免疫特异性检测对这些组分进行的详细研究表明，绵羊酪蛋白的这些次要组分可能具有与β1-和β2-酪蛋白相似的多肽链。文中还给出了一些个体乳汁中酪蛋白组分的完整电泳图谱。
