Liang Tong, Chen Mei-Jun, Zhou Ke-Yuan, Tang Xu-Dong, Wang Xu-Guang
Institute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang, Guangdong 524023, P.R. China.
Ai Zheng. 2004 Dec;23(12):1651-4.
BACKGROUND & OBJECTIVE: Nasopharyngeal carcinoma (NPC) is a common malignant tumor in southern China. Radiotherapy is a major therapy for NPC in early phase,while proper chemotherapy is necessary for NPC in late phase. Some chemotherapeutic drugs have been used to treat tumors by inducing apoptosis of tumor cells. This study was to investigate effects of curcumin on proliferation,and apoptosis of NPC cell line CNE-2Z.
CNE-2Z cells were treated with different concentrations of curcumin, inhibition rates of cell proliferation,and IC(50) of curcumin were detected by MTT method. Cell apoptosis was analyzed by flow cytometry (FCM), agarose gel electrophoresis,and Hoechst33258-PI fluorescence staining.
Proliferation of CNE-2Z cells was inhibited obviously by curcumin in dose- and time-dependent manners. IC(50) of curcumin to CNE-2Z cells at 24,48,and 72 h were (24.05+/-0.47),(19.20+/-0.17),and (7.35+/-0.50) micromol/L, respectively. After treated with 5,10,and 20 micromol/L of curcumin for 24 h, apoptotic rates of CNE-2Z cells were (4.9+/-3.2)%,(10.7+/-2.7)%, and (14.7+/-0.5)%, respectively. After treated with 10,20 micromol/L of curcumin for 24 h, morphologic changes, such as chromatin shrinkage,nuclear condensation,and chromatin fragmentation,were observed in CNE-2Z cells by fluorescent staining, a fragmented DNA ladder was detected by electrophoresis.
Curcumin may induce apoptosis, and inhibit proliferation of CNE-2Z cells.
鼻咽癌是中国南方常见的恶性肿瘤。放疗是早期鼻咽癌的主要治疗方法，而晚期鼻咽癌则需要适当的化疗。一些化疗药物通过诱导肿瘤细胞凋亡来治疗肿瘤。本研究旨在探讨姜黄素对鼻咽癌细胞系CNE-2Z增殖和凋亡的影响。
用不同浓度的姜黄素处理CNE-2Z细胞，采用MTT法检测细胞增殖抑制率和姜黄素的半数抑制浓度（IC50）。通过流式细胞术（FCM）、琼脂糖凝胶电泳和Hoechst33258-PI荧光染色分析细胞凋亡情况。
姜黄素能明显抑制CNE-2Z细胞的增殖，呈剂量和时间依赖性。姜黄素作用24、48和72小时时对CNE-2Z细胞的IC50分别为（24.05±0.47）、（19.20±0.17）和（7.35±0.50）μmol/L。用5、10和20μmol/L姜黄素处理24小时后，CNE-2Z细胞的凋亡率分别为（4.9±3.2）%、（10.7±2.7）%和（14.7±0.5）%。用10、20μmol/L姜黄素处理24小时后，荧光染色观察到CNE-2Z细胞出现染色质浓缩、核固缩和染色质断裂等形态学变化；电泳检测到DNA梯状条带。
姜黄素可能诱导CNE-2Z细胞凋亡并抑制其增殖。
