由腺病毒载体表达的严重急性呼吸综合征冠状病毒核衣壳蛋白在小鼠体内被磷酸化且具有免疫原性。

Severe acute respiratory syndrome coronavirus nucleocapsid protein expressed by an adenovirus vector is phosphorylated and immunogenic in mice.

作者信息

Zakhartchouk Alexander N, Viswanathan Sathiyanarayanan, Mahony James B, Gauldie Jack, Babiuk Lorne A

机构信息

Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3.

St Joseph's Hospital, Hamilton, ON, Canada L8N 4A6.

出版信息

J Gen Virol. 2005 Jan;86(Pt 1):211-215. doi: 10.1099/vir.0.80530-0.

DOI:10.1099/vir.0.80530-0

PMID:15604448

Abstract

Severe acute respiratory syndrome coronavirus (SARS-CoV) has been identified as the aetiological agent of SARS. Thus, vaccination against SARS-CoV may represent an effective approach towards controlling SARS. The nucleocapsid (N) protein is thought to play a role in induction of cell-mediated immunity to SARS-CoV and thus it is important to characterize this protein. In the present study, an E1/partially E3-deleted, replication-defective human adenovirus 5 (Ad5) vector (Ad5-N-V) expressing the SARS-CoV N protein was constructed. The N protein, expressed in vitro by Ad5-N-V, was of the expected molecular mass of 50 kDa and was phosphorylated. Vaccination of C57BL/6 mice with Ad5-N-V generated potent SARS-CoV-specific humoral and T cell-mediated immune responses. These results show that Ad5-N-V may potentially be used as a SARS-CoV vaccine.

摘要

严重急性呼吸综合征冠状病毒（SARS-CoV）已被确认为SARS的病原体。因此，针对SARS-CoV的疫苗接种可能是控制SARS的有效方法。核衣壳（N）蛋白被认为在诱导针对SARS-CoV的细胞介导免疫中发挥作用，因此对该蛋白进行表征很重要。在本研究中，构建了一种表达SARS-CoV N蛋白的E1/部分E3缺失、复制缺陷型人腺病毒5（Ad5）载体（Ad5-N-V）。Ad5-N-V在体外表达的N蛋白具有预期的50 kDa分子量且被磷酸化。用Ad5-N-V对C57BL/6小鼠进行疫苗接种产生了强效的SARS-CoV特异性体液免疫和T细胞介导的免疫反应。这些结果表明Ad5-N-V可能有潜力用作SARS-CoV疫苗。