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茉莉酸甲酯对人参培养物中不定根生长和人参皂苷积累的影响

Adventitious root growth and ginsenoside accumulation in Panax ginseng cultures as affected by methyl jasmonate.

作者信息

Kim Yun-Soo, Hahn Eun-Joo, Murthy Hosakatte Niranjana, Paek Kee-Yoeup

机构信息

Korea Ginseng Institute, Chung-Ang University, Ansung-shi Kyungii-do, 456-756, Korea.

出版信息

Biotechnol Lett. 2004 Nov;26(21):1619-22. doi: 10.1007/s10529-004-3183-2.

Abstract

Adventitious roots of ginseng were treated with methyl jasmonate (MJ) up to 150 microM and cultured for 40 days. Up to 100 microM MJ inhibited the root growth but increase ginsenoside accumulation. In a two-stage bioreactor culture, total ginsenosides, after elicitation with 100 microM MJ peaked after 10 days at 48 mg g(-1) dry wt and then dropped sharply. Of the two groups of ginsenosides (Rb and Rg), higher amounts of Rb accumulated in the adventitious roots.

摘要

人参不定根用高达150微摩尔的茉莉酸甲酯(MJ)处理,并培养40天。高达100微摩尔的MJ抑制根的生长,但增加人参皂苷的积累。在两阶段生物反应器培养中,用100微摩尔MJ诱导后,总人参皂苷在10天后达到峰值,为48毫克/克干重,然后急剧下降。在两组人参皂苷(Rb和Rg)中,不定根中积累了更多的Rb。

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