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从南极洲拉塞尔角盐湖分离出的新型嗜盐单胞菌菌株CRSS在复杂培养基和限定培养基上生长时产生胞外多糖。

Exopolysaccharide production by a new Halomonas strain CRSS isolated from saline lake Cape Russell in Antarctica growing on complex and defined media.

作者信息

Poli Annarita, Moriello Vincenzo Schiano, Esposito Enrico, Lama Licia, Gambacorta Agata, Nicolaus Barbara

机构信息

Istituto di Chimica Biomolecolare (ICB), CNR, Via Campi Flegrei 34, Comprensorio Olivetti, Pozzuoli (Na), 80078, Italy.

出版信息

Biotechnol Lett. 2004 Nov;26(21):1635-8. doi: 10.1007/s10529-004-3187-y.

Abstract

A haloalkalophilic Halomonas strain CRSS, isolated from salt sediments in Antarctica, produced exocellular polysaccharides (EPS) up to 2.9 g g(-1) dry cells. Acetate was the most efficient carbon source for EPS production. The composition of media strongly affected the nature of the polymers; a mannan and a xylo-mannan, were obtained when cells were grown on complex media. Acetate was the most efficient carbon source for EPS production and in presence of this substrate, a new polysaccharide, a fructo-glucan, was produced. The EPS fraction was composed by glucose, fructose, glucosamine and galactosamine in relative proportions of 1:0.7:0.3:trace.

摘要

从南极洲盐沉积物中分离出的嗜盐碱嗜盐单胞菌菌株CRSS可产生高达2.9 g g(-1)干细胞的胞外多糖(EPS)。乙酸盐是EPS生产中最有效的碳源。培养基的组成强烈影响聚合物的性质;当细胞在复合培养基上生长时,可获得一种甘露聚糖和一种木糖-甘露聚糖。乙酸盐是EPS生产中最有效的碳源,在这种底物存在的情况下,会产生一种新的多糖,即果聚糖。EPS部分由葡萄糖、果糖、氨基葡萄糖和半乳糖胺组成,相对比例为1:0.7:0.3:微量。

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