College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, 310018, People's Republic of China.
Key Laboratory of Marine Ecosystem Dynamics, Ministry of Natural Resources, Second Institute of Oceanography, Hangzhou, 310012, People's Republic of China.
Arch Microbiol. 2021 Dec 27;204(1):86. doi: 10.1007/s00203-021-02704-w.
Three yellow-pigmented, Gram-stain-negative, aerobic, motile by flagella and rod-shaped strains, designated as MC, PC and RC, were isolated from stems of Populus euphratica. Growth of those three strains occurs at 4-40 °C, pH 6.0-10.0 and with 0.5-18.0% (w/v) NaCl. Respiratory quinones contained ubiquinone-9 and ubiquione-8 as major and minor components, respectively. Major fatty acids (> 10%) were summed feature 8 (Cω6c and/or Cω7c), summed feature 3 (Cω6c and/or Cω7c) and C. Polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids, one unidentified aminolipid, one unidentified glycolipid and four unidentified lipids. Strains MC, PC and RC shared pairwise 16S rRNA gene sequence similarities of 99.9-100.0%, and showed higher similarities of 98.4-98.5% with Halomonas songnenensis NEAU-ST10-39 and 98.3-98.4% with Halomonas nanhaiensis YIM M 13059 than to other Halomonas type strains. Genomic comparisons revealed that those three strains had the pan-genome consisting of 4446 orthologous clusters, among which 676 orthologous clusters were absent in other Halomonas type strains. Phylogenomic tree indicated that strains MC, PC and RC formed an independently stable clade with Halomonas nanhaiensis YIM M 13059 and Halomonas songnenensis NEAU-ST10-39. The average nucleotide identity and digital DNA-DNA hybridization values between those three strains and other Halomonas type strains were < 89.9% and < 39.3%, respectively. Based upon phenotypic, chemotaxonomic, phylogenetic and genomic results, strains MC, PC and RC represent a novel species in the genus Halomonas, for which the name Halomonas populi sp. nov. is proposed. The type strain is MC (= JCM 33545 = MCCC 1K03942).
从胡杨茎中分离到三株黄色、革兰氏阴性、需氧、能通过鞭毛和杆状运动的菌株，分别命名为 MC、PC 和 RC。这些菌株在 4-40°C、pH 值 6.0-10.0 和 0.5-18.0%（w/v）NaCl 的条件下生长。呼吸醌含有泛醌-9 和泛醌-8 作为主要和次要成分。主要脂肪酸（>10%）为总和特征 8（Cω6c 和/或 Cω7c）、总和特征 3（Cω6c 和/或 Cω7c）和 C。极性脂包括二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、两种未鉴定的磷脂、一种未鉴定的胺脂、一种未鉴定的糖脂和四种未鉴定的脂类。菌株 MC、PC 和 RC 的 16S rRNA 基因序列相似度为 99.9-100.0%，与 Halomonas songnenensis NEAU-ST10-39 的相似度为 98.4-98.5%，与 Halomonas nanhaiensis YIM M 13059 的相似度为 98.3-98.4%，与其他 Halomonas 模式菌株的相似度更高。基因组比较表明，这三株菌具有由 4446 个直系同源簇组成的泛基因组，其中 676 个直系同源簇不存在于其他 Halomonas 模式菌株中。系统发育树表明，菌株 MC、PC 和 RC 与 Halomonas nanhaiensis YIM M 13059 和 Halomonas songnenensis NEAU-ST10-39 一起形成一个独立的稳定分支。这三株菌与其他 Halomonas 模式菌株的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为<89.9%和<39.3%。基于表型、化学分类、系统发育和基因组学结果，菌株 MC、PC 和 RC 代表了 Halomonas 属的一个新种，命名为 Halomonas populi sp. nov.。模式菌株为 MC（=JCM 33545=MCCC 1K03942）。
