通过改变喜树细胞悬浮培养中氮源供应来提高生长和喜树碱产量

Improvement of growth and camptothecin yield by altering nitrogen source supply in cell suspension cultures of Camptotheca acuminata.

作者信息

Pan Xue-Wu, Xu Heng-Hao, Liu Xin, Gao Xiang, Lu Ying-Tang

机构信息

Key Lab of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan, Hubei Province 430072, P.R. China.

出版信息

Biotechnol Lett. 2004 Nov;26(22):1745-8. doi: 10.1007/s10529-004-4580-2.

DOI:10.1007/s10529-004-4580-2

PMID:15604829

Abstract

Nitrate at 70 mM gave the highest biomass of Camptotheca acuminata in suspension culture in MS medium, but a NH4+/NO3- molar ratio of 5:1 (giving a total of 40 mM N) gave the maximum camptothecin yield. A two-stage flask culture system was established to improve culture efficiency; cell dry weight, camptothecin content and yield was increased by 30%, 280% and 340%, respectively when compared with those of control, reaching up to 36 g l(-1), 0.36 mg g(-1), and 12.8 mg l(-1), respectively.

摘要

在MS培养基的悬浮培养中，70 mM的硝酸盐使喜树产生的生物量最高，但NH4+/NO3-摩尔比为5:1（总氮量为40 mM）时喜树碱产量最高。建立了两阶段摇瓶培养系统以提高培养效率；与对照相比，细胞干重、喜树碱含量和产量分别提高了30%、280%和340%，分别达到36 g l(-1)、0.36 mg g(-1)和12.8 mg l(-1)。