[用薄层扫描法研究喜树果中喜树碱的动态积累]
[The study of dynamic acccumulation of camptothecin in Camptotheca acuminata fruit by TLCS].
作者信息
Jiang Ming-Lian, Huang Rui-Song, Wu Yi, Zhou Fang-Li, Su Qing, Wei Ai-Yan
机构信息
Nanning Institute for Food and Drug Control, Nanning 530001, China.
出版信息
Zhong Yao Cai. 2008 May;31(5):684-6.
OBJECTIVE
To establish TLC scanning method for the determination of camptothecin in Camptotheca acuminata fruit, and analysis the dynamic accumulation of camptothecin in Camptotheca acuminata fruit to find out the best collection period.
METHODS
Silica gel H-CMC-Na thin layer plate was adopted in the determination with chloroform-acetone (7 : 3) used as deeloper, Single-wavelength and linear scanning of TLC was used, and the detection wavelength was 360 nm.
RESULTS
There was a good linear relationship for Comptothecin within the range of 0.0542 - 0.3252 microg, the average recovery was 97.13%, RSD was 1.76%.
CONCLUSION
The method is accurate, simple and reliable, and can be used for the determination of camptothecin in Camptotheca acuminata fruit and dynamic accumulation research.
目的
建立薄层层析扫描法测定喜树果中喜树碱的含量，并分析喜树果中喜树碱的动态积累情况，以找出最佳采收期。
方法
采用硅胶H-CMC-Na薄层层析板，以氯仿-丙酮（7∶3）为展开剂进行测定，薄层层析采用单波长线性扫描，检测波长为360nm。
结果
喜树碱在0.0542～0.3252μg范围内线性关系良好，平均回收率为97.13%，RSD为1.76%。
结论
该方法准确、简便、可靠，可用于喜树果中喜树碱的含量测定及动态积累研究。
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