牛病毒性腹泻病毒通过网格蛋白依赖性受体介导的内吞作用被内化。

Bovine viral diarrhoea virus is internalized by clathrin-dependent receptor-mediated endocytosis.

作者信息

Grummer B, Grotha S, Greiser-Wilke I

机构信息

Department of Infectious Diseases, Institute for Virology, University of Veterinary Medicine Hannover, Foundation Buenteweg 17, 30559 Hannover, Germany.

出版信息

J Vet Med B Infect Dis Vet Public Health. 2004 Dec;51(10):427-32. doi: 10.1111/j.1439-0450.2004.00798.x.

DOI:10.1111/j.1439-0450.2004.00798.x

PMID:15606865

Abstract

Bovine viral diarrhoea virus (BVDV) is a pestivirus within the family Flaviviridae. In contrast to the members of the genus flavivirus, nothing is known about the viral entry route for pestiviruses. In this study, the process of BVDV infection following attachment to the cell surface was examined. BVDV clearly co-localizes with clathrin, with early endosome antigen-1 (EEA-1), an early endosome marker, and also with lysosomal-associated membrane protein-2 (LAMP-2), a lysosomal marker. BVDV internalization is inhibited by compounds that block clathrin- but not caveolae-dependent endocytosis. These findings demonstrate that BVDV enters the cells via the clathrin-coated pit pathway.

摘要

牛病毒性腹泻病毒（BVDV）是黄病毒科瘟病毒属的一种病毒。与黄病毒属的成员不同，关于瘟病毒的病毒进入途径尚无任何了解。在本研究中，对BVDV附着于细胞表面后的感染过程进行了检查。BVDV明显与网格蛋白、早期内体标记物早期内体抗原-1（EEA-1）以及溶酶体标记物溶酶体相关膜蛋白-2（LAMP-2）共定位。BVDV的内化受到阻断网格蛋白介导而非小窝蛋白介导的内吞作用的化合物的抑制。这些发现表明BVDV通过网格蛋白包被小窝途径进入细胞。