Department of Virology, National Veterinary Research Institute, 24-100 Pulawy, Poland.
Department of Biochemistry, National Veterinary Research Institute, 24-100 Pulawy, Poland.
Viruses. 2022 Jun 11;14(6):1276. doi: 10.3390/v14061276.
Bovine viral diarrhea virus (BVDV) belongs to the family and the genus. Infection with BVDV causes a disease with a wide spectrum of clinical symptoms, most often mild, although infections with this virus constitute a serious economic problem all over the world. The virus is characterized by a high genetic variability, while the accumulation of single mutations leads to the formation of its new variants. The aim of this study was to better understand the complicated pathogenesis of this disease at the molecular level via the analysis of the transcriptome of cells infected with this virus. The bovine kidney cell line (MDBK), the cytopathic (cp) reference strain, and two non-cytopathic (ncp) BVD virus field strains were used in transcriptomic studies. The cell transcriptome was tested 24 and 72 h after infection. The results of the microarray analysis revealed changes in the expression levels of numerous genes. Genes with changed expression as a result of infection with the cp strain caused changes in the expression levels of a large number of genes and enriched a number of pathways. Genes with increased expression levels were enriched among other pathways involved in the cell cycle, while genes with reduced expression levels enriched pathways mostly related to metabolism. Genes with increased expression levels as a result of infection with ncp strains enriched a much smaller number of pathways, among them, pathways related to signaling activity 24 h post-infection and serine biosynthetic pathways both 24 and 72 h post-infection. Pathways enriched by genes with reduced expression levels were related to the innate immune response (72 h post-infection) or metabolism (24 and 72 h post-infection). The results of microarray studies can help us to better understand the host's response to BVDV infection.
牛病毒性腹泻病毒（BVDV）属于 科和 属。BVDV 的感染会引起一系列临床表现，大多数情况下症状较轻，但这种病毒的感染在全球范围内构成了严重的经济问题。该病毒的特点是具有高度遗传变异性，而单个突变的积累会导致其新变体的形成。本研究旨在通过分析感染该病毒的细胞的转录组，从分子水平更好地了解这种疾病的复杂发病机制。本研究使用牛肾细胞系（MDBK）、细胞病变（cp）参考株和两种非细胞病变（ncp）BVD 病毒田间株进行转录组研究。在感染后 24 和 72 小时检测细胞转录组。微阵列分析的结果显示了许多基因表达水平的变化。感染 cp 株导致大量基因表达水平的变化，并富集了许多途径。表达水平发生变化的基因在细胞周期相关途径中富集，而表达水平降低的基因则主要富集在代谢相关途径中。感染 ncp 株后表达水平升高的基因富集的途径要少得多，其中包括感染后 24 小时的信号转导活性途径和感染后 24 和 72 小时的丝氨酸生物合成途径。表达水平降低的基因富集的途径与先天免疫反应（感染后 72 小时）或代谢（感染后 24 和 72 小时）有关。微阵列研究的结果可以帮助我们更好地理解宿主对 BVDV 感染的反应。
