用于测定滤纸上干燥全血样本中乙胺嘧啶的简单且经济高效的液相色谱法。
Simple and cost-effective liquid chromatographic method for determination of pyrimethamine in whole blood samples dried on filter paper.
作者信息
Minzi O M S, Massele A Y, Gustafsson L L, Ericsson O
机构信息
Medicinal Chemistry Department, School of Pharmacy, Muhimbili University College of Health Sciences, P.O. Box 65013, Dar Es Salaam, Tanzania.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jan 5;814(1):179-83. doi: 10.1016/j.jchromb.2004.10.049.
A cost-effective HPLC method for determination of pyrimethamine (PYR) in human whole blood samples dried on filter paper (Whatman) is reported. Trimethoprim (TMP) was used as an internal standard. Whole blood spiked with PYR was transferred (100 microl) onto filter paper and dried at room temperature. Capillary blood samples (100 microl) after ingestion of three tablets of sulfadoxine-pyrimethamine (SP) by one subject were also tested. PYR and an internal standard (IS) TMP were extracted into di-isopropyl ether as bases and then re-extracted with 150 microl mobile phase. A C-18 column was used and the mobile phase consisted of phosphate buffer (0.05 M, pH 5):acetonitrile:concentrated perchloric acid (750:300:2.5, v/v/v). The absorbances of PYR and IS were monitored at 270 nm. The limit of quantification was 40 ng/ml. The within- and between-assay coefficient of variations were <10% at the limit of quantification.
报道了一种经济高效的高效液相色谱法，用于测定滤纸上（沃特曼）干燥的人全血样本中的乙胺嘧啶（PYR）。甲氧苄啶（TMP）用作内标。将加有PYR的全血（100微升）转移到滤纸上，并在室温下干燥。还对一名受试者摄入三片磺胺多辛-乙胺嘧啶（SP）后的毛细血管血样本（100微升）进行了测试。PYR和内标（IS）TMP以碱的形式萃取到二异丙醚中，然后用150微升流动相进行再萃取。使用C-18柱，流动相由磷酸盐缓冲液（0.05M，pH5）：乙腈：高氯酸（750:300:2.5，v/v/v）组成。在270nm处监测PYR和IS的吸光度。定量限为40ng/ml。在定量限处，批内和批间变异系数均<10%。
Zotero 插件