Bell D J, Nyirongo S K, Molyneux M E, Winstanley P A, Ward S A
Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Mar 1;847(2):231-6. doi: 10.1016/j.jchromb.2006.10.020. Epub 2006 Nov 13.
This article describes high-performance liquid chromatographic assays for the quantification of sulfadoxine (SDX), pyrimethamine (PYM), chloroquine (CQ), amodiaquine (AQ) and desethylamodiaquine (AQM) from whole blood. All four assays were set up and validated in Malawi using a common high-performance liquid chromatography platform and column and involved the use of simple mobile phase and extraction reagents. Calibration curves were linear (r(2)>0.95) in the ranges 5-100microg/ml, 50-1000, 150-1500, 100-1000 and 100-1000ng/ml for SDX, PYM, CQ, AQ and AQM, respectively. Intra-assay and inter-assay coefficients of variation were <15% at 3 points spanning the concentration range and <20% at the lower limit of quantification. The assays were specific with no interference from the other antimalarials described in this report. All four assays use liquid-liquid extraction, reversed-phase chromatography and UV detection and require between 50 and 200microl of blood. Because the assays share common instruments and reagents, they are cost-efficient and could be used to optimise antimalarial drug therapies in other resource poor settings.
本文介绍了用于定量全血中磺胺多辛（SDX）、乙胺嘧啶（PYM）、氯喹（CQ）、阿莫地喹（AQ）和去乙基阿莫地喹（AQM）的高效液相色谱法。所有这四种方法均在马拉维使用通用的高效液相色谱平台和色谱柱建立并验证，涉及使用简单的流动相和萃取试剂。磺胺多辛、乙胺嘧啶、氯喹、阿莫地喹和去乙基阿莫地喹的校准曲线在5 - 100μg/ml、50 - 1000、150 - 1500、100 - 1000和100 - 1000ng/ml范围内呈线性（r²>0.95）。在跨越浓度范围的3个点处，批内和批间变异系数<15%，在定量下限处<20%。这些方法具有特异性，不受本报告中所述其他抗疟药的干扰。所有这四种方法均采用液 - 液萃取、反相色谱法和紫外检测，需要50至200μl血液。由于这些方法共享通用的仪器和试剂，它们具有成本效益，可用于在其他资源匮乏地区优化抗疟药物治疗。
