Chen Jun, Cassar Steven C, Zhang Di, Gopalakrishnan Murali
Neuroscience Research, Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, IL 60064-6125, USA.
Biochem Biophys Res Commun. 2005 Jan 28;326(4):887-93. doi: 10.1016/j.bbrc.2004.11.125.
Kcv, the first identified viral potassium channel encoded by the green algae Paramecium bursaria chlorella virus (PBCV-1), conducted K(+) selective currents when expressed in heterologous systems. This K(+) channel was proposed to be important for PBCV-1 infection and replication. In the present study, we identified and functionally characterized a novel K(+) channel Kesv, encoded by Ectocarpus siliculosus virus that infects filamentous marine brown algae. Kesv encodes a protein of 124 amino acids and is 21.8% identical and 37.1% homologous to Kcv. Membrane topology programs predicted that Kesv consists of three transmembrane domains. When expressed in Xenopus oocytes, Kesv induced largely instantaneous, K(+) selective currents that were sensitive to block by Ba(2+) and amantadine. Thus, Kesv along with Kcv, constitutes an emerging family of viral potassium channels, which may play important roles in the life cycle of viruses.
Kcv是首个被鉴定出的由绿藻草履虫小球藻病毒（PBCV-1）编码的病毒钾通道，在异源系统中表达时传导K⁺选择性电流。该K⁺通道被认为对PBCV-1的感染和复制很重要。在本研究中，我们鉴定并对一种新型K⁺通道Kesv进行了功能表征，它由感染丝状海洋褐藻的硅藻病毒编码。Kesv编码一个124个氨基酸的蛋白质，与Kcv的同一性为21.8%，同源性为37.1%。膜拓扑结构程序预测Kesv由三个跨膜结构域组成。当在非洲爪蟾卵母细胞中表达时，Kesv诱导出主要为瞬时性的K⁺选择性电流，该电流对Ba²⁺和金刚烷胺的阻断敏感。因此，Kesv与Kcv一起构成了一个新出现的病毒钾通道家族，它们可能在病毒的生命周期中发挥重要作用。
