Salik E, Ercan F, Sirvanci S, Cetinel S, Onat F, San T
Marmara University, School of Medicine, Department of Histology and Embryology, Haydarpaşa, 34668 Istanbul, Turkey.
Brain Res Bull. 2005 Jan 15;64(5):409-15. doi: 10.1016/j.brainresbull.2004.09.010.
Hippocampal formation is extremely sensitive to the aging process and appears to be one of the first regions to show structural and physiological changes with advancing age. Basic fibroblast growth factor (bFGF) plays an important role in the stimulation of mitogenesis in glial cells, the support of neuronal survival and the promotion of neurite outgrowth in vitro. In the present study, the effect of aging on the distribution of bFGF immunoreactive (bFGF-ir) cells was investigated. The protein product of bFGF was visualized immunohistochemically in the dorsal hippocampus of Wistar albino rats. bFGF-ir astrocytes in different subfields of hippocampus and neurons in CA2 field were quantified to determine whether changes in immunoreactivity were correlated with advancing age. Aging was accompanied by a decrease in bFGF-ir cell density in subfields of hippocampus. We concluded that aging was associated with a reduction in bFGF-ir cell density that may reflect a decreased expression of bFGF in the rat hippocampus.
海马结构对衰老过程极为敏感，似乎是随着年龄增长最早出现结构和生理变化的脑区之一。碱性成纤维细胞生长因子（bFGF）在刺激神经胶质细胞有丝分裂、支持神经元存活以及促进体外神经突生长方面发挥着重要作用。在本研究中，我们调查了衰老对bFGF免疫反应性（bFGF-ir）细胞分布的影响。通过免疫组织化学方法在Wistar白化大鼠的背侧海马中观察bFGF的蛋白质产物。对海马不同亚区的bFGF-ir星形胶质细胞和CA2区的神经元进行定量分析，以确定免疫反应性的变化是否与年龄增长相关。衰老伴随着海马亚区bFGF-ir细胞密度的降低。我们得出结论，衰老与bFGF-ir细胞密度的降低有关，这可能反映了大鼠海马中bFGF表达的减少。
