Pozdniakovaite Natalija, Popendikyte Violeta
Institute of Biotechnology, V. Graiciuno 8, Vilnius, LT-02241, Lithuania.
Dev Growth Differ. 2004 Dec;46(6):545-54. doi: 10.1111/j.1440-169x.2004.00771.x.
We used differential display analysis of mRNA to investigate the differences between gene expression in wild-type (wt) yeast Saccharomyces cerevisiae cells and mutated ones with disrupted activity of genes MMF1 and HMF1, members of the YERO57c/YJGF family. Reverse transcription-polymerase chain reaction (RT-PCR) analysis was performed to determine the differences in the degree of expression of 14 specific transcripts in normal and mutated yeast cells. Obtained data demonstrate that disruption of genes encoding proteins Mmf1p, Hmf1p (or both of them) result in the correlative variation of expression level of the target 12 genes both in the cells with changed phenotype (mmf1 and mmf1 hmf1) and in the cells retaining w.t. shape and growth rate (wt cells, hmf1). Metabolic processes and cellular pathways have been indicated for Mmf1p and Hmf1p based on the different profiles of the expression of 14 genes in mmf1, hmf1 yeast S. cerevisiae cells.
我们利用mRNA差异显示分析法，研究野生型酿酒酵母细胞与YERO57c/YJGF家族成员MMF1和HMF1基因活性被破坏的突变细胞之间的基因表达差异。通过逆转录聚合酶链反应（RT-PCR）分析，确定正常酵母细胞和突变酵母细胞中14种特定转录本的表达程度差异。所得数据表明，编码蛋白质Mmf1p、Hmf1p（或两者）的基因破坏，会导致目标12个基因的表达水平在表型改变的细胞（mmf1和mmf1 hmf1）以及保持野生型形状和生长速率的细胞（野生型细胞、hmf1）中发生相关变化。基于mmf1、hmf1酿酒酵母细胞中14个基因的不同表达谱，已指出Mmf1p和Hmf1p的代谢过程及细胞途径。
