Loeb E
Faculty of Veterinary Medicine, Department of Pathobiology, Utrecht University, PO Box 80158, NL-3508 TD, Utrecht, The Netherlands.
J Vet Med A Physiol Pathol Clin Med. 2004 Dec;51(9-10):453-5. doi: 10.1111/j.1439-0442.2004.00656.x.
A retrospective analysis of 42 ruminants (sheep, goats and cattle) with suspected meningo-encephalitis was performed. The clinical findings and the post-mortem results of the animals have been specified. Bacteriological culture, Gram's stain and Listeria-specific immunohistochemistry were performed in order to confirm the diagnosis of these cases. The results of the different methods were evaluated for the detection of listerial antigens. Bacteriological culture was positive in 28.5% of the cases. In 47.6% of the cases, Gram-positive bacteria were found and 80.9% of the cases were immunohistochemically positive for the listerial antigen. The most important conclusion from this investigation is that the traditionally used Gram's stain and the bacteriological culture techniques are insufficient compared with immunohistochemistry for the confirmation of a Listeria monocytogenes infection.
对42只疑似脑膜脑炎的反刍动物（绵羊、山羊和牛）进行了回顾性分析。已详细说明这些动物的临床症状和尸检结果。为确诊这些病例，进行了细菌培养、革兰氏染色和李斯特菌特异性免疫组织化学检测。评估了不同方法检测李斯特菌抗原的结果。28.5%的病例细菌培养呈阳性。47.6%的病例发现革兰氏阳性菌，80.9%的病例李斯特菌抗原免疫组织化学检测呈阳性。本次调查最重要的结论是，与免疫组织化学相比，传统使用的革兰氏染色和细菌培养技术在确诊单核细胞增生李斯特菌感染方面存在不足。
