[尼古丁对胚胎干细胞Oct-4转录的影响]
[Effect of nicotine on Oct-4 transcription of embryonic stem cells].
作者信息
Zhang Huaping, Guo Dongxing, Zhao Yangxing, Wang Lianyun, Qia Zhongdong
机构信息
Division of Parasitology, Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
出版信息
Wei Sheng Yan Jiu. 2004 Sep;33(5):547-9.
UNLABELLED
To clarify whether nicotine affected the early embryo development, the paper investigated the influence of nicotine on embryonic stem (ES) cells specific gene Oct-4.
METHODS
ES cells were treated with nicotine (1-1000 nM) and/or 10 microM tubocurarine (a usual nicotinic acetylcholine receptors [nAChRs] blocker) for 24 h. Subsequently, they were purified to discard feeder cells and total RNA was isolated. Fgf-5 was amplified to detect the purification of ES cells, and the relative expression of Oct-4 and beta-actin to GAPDH was analyzed with RT-PCR.
RESULTS
RT-PCR analysis illustrated that nicotine (10-1000 nM) significantly enhanced Oct-4 transcription, while had no effect on beta-actin transcription; meanwhile, compared with nicotine (100 nM and 1000 nM) treatment alone, tubocurarine inhibited Oct-4 transcription evidently.
CONCLUSION
It is reasonable to assume that nicotine could influence the development and differentiation of ES cells, and impinge on the early embryo development.
未标注
为阐明尼古丁是否影响早期胚胎发育，本文研究了尼古丁对胚胎干细胞（ES细胞）特异性基因Oct-4的影响。
方法
将ES细胞用尼古丁（1 - 1000 nM）和/或10 μM筒箭毒碱（一种常用的烟碱型乙酰胆碱受体[nAChRs]阻滞剂）处理24小时。随后，将它们纯化以去除饲养细胞并分离总RNA。扩增Fgf-5以检测ES细胞的纯化情况，并用RT-PCR分析Oct-4和β-肌动蛋白相对于GAPDH的相对表达。
结果
RT-PCR分析表明，尼古丁（10 - 1000 nM）显著增强Oct-4转录，而对β-肌动蛋白转录无影响；同时，与单独用尼古丁（100 nM和1000 nM）处理相比，筒箭毒碱明显抑制Oct-4转录。
结论
可以合理推测尼古丁可能影响ES细胞的发育和分化，并影响早期胚胎发育。
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