Zhang Huaping, Guo Dongxing, Zhao Yangxing, Wang Lianyun, Qia Zhongdong
Division of Parasitology, Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Wei Sheng Yan Jiu. 2004 Sep;33(5):547-9.
To clarify whether nicotine affected the early embryo development, the paper investigated the influence of nicotine on embryonic stem (ES) cells specific gene Oct-4.
ES cells were treated with nicotine (1-1000 nM) and/or 10 microM tubocurarine (a usual nicotinic acetylcholine receptors [nAChRs] blocker) for 24 h. Subsequently, they were purified to discard feeder cells and total RNA was isolated. Fgf-5 was amplified to detect the purification of ES cells, and the relative expression of Oct-4 and beta-actin to GAPDH was analyzed with RT-PCR.
RT-PCR analysis illustrated that nicotine (10-1000 nM) significantly enhanced Oct-4 transcription, while had no effect on beta-actin transcription; meanwhile, compared with nicotine (100 nM and 1000 nM) treatment alone, tubocurarine inhibited Oct-4 transcription evidently.
It is reasonable to assume that nicotine could influence the development and differentiation of ES cells, and impinge on the early embryo development.
为阐明尼古丁是否影响早期胚胎发育，本文研究了尼古丁对胚胎干细胞（ES细胞）特异性基因Oct-4的影响。
将ES细胞用尼古丁（1 - 1000 nM）和/或10 μM筒箭毒碱（一种常用的烟碱型乙酰胆碱受体[nAChRs]阻滞剂）处理24小时。随后，将它们纯化以去除饲养细胞并分离总RNA。扩增Fgf-5以检测ES细胞的纯化情况，并用RT-PCR分析Oct-4和β-肌动蛋白相对于GAPDH的相对表达。
RT-PCR分析表明，尼古丁（10 - 1000 nM）显著增强Oct-4转录，而对β-肌动蛋白转录无影响；同时，与单独用尼古丁（100 nM和1000 nM）处理相比，筒箭毒碱明显抑制Oct-4转录。
可以合理推测尼古丁可能影响ES细胞的发育和分化，并影响早期胚胎发育。
