Ireland H Elyse, Clutterbuck Andy, Cloquet Jean-Phillipe, Thurston Milo I, Williams Peter A, Cronk Quentin C, Dewey F Molly, Williams John H H
Chester Centre for Stress Research, Department of Biological Sciences, University College Chester, Parkgate Road, Chester, CH1 4BJ, United Kingdom.
J Agric Food Chem. 2004 Dec 29;52(26):7804-8. doi: 10.1021/jf048864h.
Gum arabic from Acacia senegal is commonly used as an additive in foodstuffs. Adulteration of gum arabic by other gums is a potential problem for reasons of safety and quality. This study aimed to develop and evaluate the use of enzyme-linked immunosorbent assays (ELISAs) for the detection of potential adulterants of gum arabic. Indirect competitive ELISAs (IC-ELISAs) were developed using the monoclonal antibodies SY CC7 (A. senegal), SY HH3 (Acacia seyal), and SY J1A1 (Combretum erythrophyllum). All IC-ELISAs had a working range of 0.005-10 mg/mL. The antibodies used were tested using the IC-ELISAs for cross-reactivity with other Acacia species and other gums. The antibodies were very specific for their respective antigens. Significant cross-reactivity was found for SY CC7 (between A. senegal and A. melliferae) and SY J1A1 (between C. erythrophyllum and A. seyal). The IC-ELISA was adapted further to test confectionery samples for the presence of gum arabic, which was successful, although recovery rates were reduced. Both IC- and plate trapped antigen ELISA (PTA-ELISA) formats were able to distinguish an adulterated sample of gum arabic when blended with either A. seyal or C. erythrophyllum. The PTA-ELISA was more sensitive for A. seyal than the IC-ELISA, but both were equally sensitive for C. erythrophyllum. The results suggest that the antibodies SY CC7, SY HH3, and SY J1A1 could be used in combination with each other for the detection of potential adulterants of A. senegal and the detection of gum arabic in foodstuffs.
来自塞内加尔金合欢的阿拉伯胶通常用作食品添加剂。由于安全和质量原因，用其他树胶掺假阿拉伯胶是一个潜在问题。本研究旨在开发和评估酶联免疫吸附测定法（ELISA）用于检测阿拉伯胶潜在掺假物。使用单克隆抗体SY CC7（塞内加尔金合欢）、SY HH3（阿拉伯胶树）和SY J1A1（红褐风车子）开发了间接竞争ELISA（IC-ELISA）。所有IC-ELISA的工作范围为0.005-10 mg/mL。使用IC-ELISA测试所用抗体与其他金合欢属物种和其他树胶的交叉反应性。这些抗体对其各自的抗原具有高度特异性。发现SY CC7（塞内加尔金合欢和蜜蜂蜜蜂之间）和SY J1A1（红褐风车子和阿拉伯胶树之间）存在显著交叉反应。进一步调整IC-ELISA以测试糖果样品中阿拉伯胶的存在，结果成功，尽管回收率有所降低。IC-ELISA和平板捕获抗原ELISA（PTA-ELISA）两种形式都能够区分与阿拉伯胶树或红褐风车子混合的掺假阿拉伯胶样品。PTA-ELISA对阿拉伯胶树的检测比IC-ELISA更敏感，但两者对红褐风车子的敏感性相同。结果表明，抗体SY CC7、SY HH3和SY J1A1可以相互结合使用，用于检测塞内加尔金合欢的潜在掺假物以及食品中阿拉伯胶的检测。
