Frederix Sofie A, Van Hoeymissen Klaartje E, Courtin Christophe M, Delcour Jan A
Laboratory of Food Chemistry, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, 3001 Leuven, Belgium.
J Agric Food Chem. 2004 Dec 29;52(26):7950-6. doi: 10.1021/jf049041v.
Water-extractable arabinoxylan (WE-AX) of variable molecular weight (MW) and water-unextractable arabinoxylan (WU-AX) were added to wheat flour to study their effect on gluten agglomeration in a dough and batter gluten-starch separation process with recovery of gluten from the batter with a set of vibrating sieves (400, 250, and 125 microm). Low MW WE-AX had almost no impact on the distribution of the gluten on the different sieves. High MW WE-AX decreased yields of the largest (400 microm sieve) gluten aggregates, more than their medium MW counterparts, indicating the importance of AX MW for their effect on gluten interactions. Correlations between the total level of gluten protein recovered on the three sieves and the batter extract viscosity as well as between the proportion of gluten protein recovered on the 400 microm sieve to that on the three sieves and the batter extract viscosity pointed to the importance of viscosity as an indicator for gluten agglomeration, as did the fact that another viscosity increasing plant polysaccharide (guar gum) also negatively influenced gluten agglomeration. However, the obtained data cannot rule out that AX and guar gum also exert steric effects on gluten agglomeration. WU-AX, present as discrete cell wall fragments, had a negative impact on the level of large gluten aggregates. Taken together, the results show that both native WE-AX and WU-AX detrimentally impact gluten agglomeration.
将不同分子量（MW）的水可提取阿拉伯木聚糖（WE-AX）和水不可提取阿拉伯木聚糖（WU-AX）添加到小麦粉中，以研究它们在面团和面糊中面筋 - 淀粉分离过程中对面筋团聚的影响，并通过一组振动筛（400、250和125微米）从面糊中回收面筋。低分子量的WE-AX对不同筛网上面筋的分布几乎没有影响。高分子量的WE-AX比中等分子量的WE-AX更能降低最大（400微米筛）面筋聚集体的产量，这表明阿拉伯木聚糖的分子量对其面筋相互作用的影响很重要。在三个筛网上回收的面筋蛋白总量与面糊提取物粘度之间的相关性，以及在400微米筛上回收的面筋蛋白占三个筛网上回收的面筋蛋白的比例与面糊提取物粘度之间的相关性，都表明粘度作为面筋团聚指标的重要性，另一种增加粘度的植物多糖（瓜尔胶）也对面筋团聚产生负面影响这一事实也证明了这一点。然而，获得的数据不能排除阿拉伯木聚糖和瓜尔胶对面筋团聚也有空间效应。以离散细胞壁碎片形式存在的WU-AX对大面筋聚集体的水平有负面影响。综上所述，结果表明天然的WE-AX和WU-AX均对面筋团聚有不利影响。
