Peacock Z S, Barnes L A, King W F, Trantolo D J, Wise D L, Taubman M A, Smith D J
Department of Immunology, The Forsyth Institute, Boston, MA 02115, USA.
Oral Microbiol Immunol. 2005 Feb;20(1):60-4. doi: 10.1111/j.1399-302X.2004.00191.x.
Subcutaneous immunization with SYI, a peptide construct based on Streptococcus mutans glucan binding protein B (GbpB) residues 113-132, significantly reduces experimental dental caries. Since mucosal immunization may be preferred for human vaccine applications, the present objective was to determine what formulation of SYI combined with polylactide-coglycolide microparticles could give rise to significant levels of salivary IgA antibody reactive with the native GbpB protein. A comparison of the SYI construct, loaded into or mixed with polylactide-coglycolide revealed the SYI-loaded microparticles to induce significant and sustainable levels of salivary and nasal wash IgA antibody to the peptide and the native protein. SYI mixed with unloaded microparticles was less effective in mucosal antibody response induction. These studies indicate that mucosal immunization with the SYI construct can induce salivary IgA antibody to a pathogenesis-associated component of S. mutans if delivered within polylactide-coglycolide microparticles, suggesting that this approach could successfully induce protective salivary immunity to dental caries caused by S. mutans.
用基于变形链球菌葡聚糖结合蛋白B（GbpB）第113 - 132位残基的肽构建体SYI进行皮下免疫，可显著降低实验性龋齿。由于黏膜免疫可能更适合人类疫苗应用，目前的目标是确定SYI与聚丙交酯 - 乙交酯微粒联合使用的何种制剂能够产生与天然GbpB蛋白反应的高水平唾液IgA抗体。对载入或混入聚丙交酯 - 乙交酯中的SYI构建体进行比较发现，载入SYI的微粒可诱导唾液和鼻腔灌洗液中针对该肽和天然蛋白的IgA抗体达到显著且可持续的水平。与未载入微粒的SYI混合在诱导黏膜抗体反应方面效果较差。这些研究表明，如果在聚丙交酯 - 乙交酯微粒中递送，用SYI构建体进行黏膜免疫可诱导针对变形链球菌致病相关成分的唾液IgA抗体，这表明该方法可成功诱导对变形链球菌所致龋齿的保护性唾液免疫。
