Ji X, Zhang Q, Liu Y
College of Life Sciences, Peking University, Beijing, People's Republic of China.
Protoplasma. 2004 Dec;224(3-4):211-6. doi: 10.1007/s00709-004-0065-3. Epub 2004 Dec 22.
Epifluorescence microscopy of mature pollen grains of Turnera ulmifolia and Zantedeschia aethiopica stained with 4',6-diamidino-2-phenylindole demonstrated the presence of fluorescent cytoplasmic DNA aggregates in the male reproductive cells of both species. Double staining of the cells with 4',6-diamidino-2-phenylindole and 3,3'-dihexyloxacarbocyanine iodide in Technovit resin sections showed that the mitochondria of these cells did not correspond to the fluorescent cytoplasmic DNA aggregates. Electron microscopy studies revealed both plastids and mitochondria in the cells of these species. In addition, immunoelectron microscopy using an anti-DNA monoclonal antibody showed clear labeling of plastids but not mitochondria. These data provide cytological evidence for biparental plastid inheritance and maternal mitochondrial inheritance in these species.
用4',6-二脒基-2-苯基吲哚染色的榆叶茑萝和马蹄莲成熟花粉粒的落射荧光显微镜检查表明，这两个物种的雄性生殖细胞中均存在荧光细胞质DNA聚集体。在Technovit树脂切片中用4',6-二脒基-2-苯基吲哚和3,3'-二己基氧杂羰花青碘化物对细胞进行双重染色显示，这些细胞的线粒体与荧光细胞质DNA聚集体不对应。电子显微镜研究揭示了这些物种细胞中的质体和线粒体。此外，使用抗DNA单克隆抗体的免疫电子显微镜检查显示质体有明显标记，而线粒体没有。这些数据为这些物种中双亲质体遗传和母系线粒体遗传提供了细胞学证据。
