Chung Chih-Yuan, Yeh Kun-Tu, Hsu Nicholas C, Chang Julia Huei-Mei, Lin Jen-Tsun, Horng Huey-Ching, Chang Cheng-Shyong
Division of Hematology-Oncology, Department of Internal Medicine, Changhua Christian Hospital, 135 Nanhsiao Street, Changhua 500, Taiwan, ROC.
Cancer Lett. 2005 Jan 20;217(2):231-6. doi: 10.1016/j.canlet.2004.06.045.
The expression of the c-kit protooncogene in human hepatocellular carcinoma (HCC) was investigated. Immunohistochemical staining (IHC) and RT-PCR were employed to examine the protein and mRNA expression of c-kit protooncogene, respectively. IHC results demonstrated that 22 of 86 (25.6%) HCC tissue sections expressed c-kit protein. The c-kit mRNA transcript was further confirmed in all 22 IHC c-kit positive HCC tissue samples by RT-PCR. Moreover, the relationship between c-kit expression in HCC and prognosis of patients was statistically analyzed and a correlation was established. The group of patients whose HCC specimens showed positive c-kit staining exhibited better survival as compared to those patients with negative c-kit expression (p=0.021). These results suggest that c-kit expression may be a good prognostic indicator for HCC.
研究了c-kit原癌基因在人类肝细胞癌（HCC）中的表达情况。分别采用免疫组织化学染色（IHC）和逆转录聚合酶链反应（RT-PCR）检测c-kit原癌基因的蛋白质和mRNA表达。免疫组织化学染色结果显示，86例HCC组织切片中有22例（25.6%）表达c-kit蛋白。通过RT-PCR在所有22例免疫组织化学染色c-kit阳性的HCC组织样本中进一步证实了c-kit mRNA转录本。此外，对HCC中c-kit表达与患者预后的关系进行了统计学分析并建立了相关性。与c-kit表达阴性的患者相比，HCC标本显示c-kit染色阳性的患者组生存期更长（p = 0.021）。这些结果表明，c-kit表达可能是HCC的一个良好预后指标。
