Wharton David A, Mutch Jodi S, Wilson Peter W, Marshall Craig J, Lim Miang
Department of Zoology, University of Otago, P.O. Box 56, Dunedin, New Zealand.
Cryo Letters. 2004 Sep-Oct;25(5):335-40.
The construction of a simple ice nucleation spectrometer is described. It uses 10 microliter droplets loaded into glass capillary tubes which are then inserted into an aluminium holder. Each holder takes six capillary tubes surrounding a central thermocouple. Four holders are placed into a cooling block, cooled by fluid from a programmable refrigerated circulator, and the thermocouples interfaced to a computer to record temperatures. Freezing of each sample is detected by an exotherm on the temperature recording, with 24 samples recorded per run. The spectrometer was tested using deionized water, an extract from a New Zealand alpine cockroach and an extract of lawn grass. The cockroach extract is estimated to contain about 10(3) more nucleators, active at -5 degrees C, than the grass extract.
本文描述了一种简易冰核光谱仪的构造。它使用装载在玻璃毛细管中的10微升液滴，然后将毛细管插入铝制支架中。每个支架容纳围绕中心热电偶的六个毛细管。四个支架放置在一个冷却块中，由可编程制冷循环器的流体冷却，热电偶连接到计算机以记录温度。通过温度记录上的放热检测每个样品的冻结，每次运行记录24个样品。使用去离子水、新西兰高山蟑螂提取物和草坪草提取物对该光谱仪进行了测试。据估计，蟑螂提取物在-5摄氏度下比草提取物含有多约10³个活性成核剂。
