[丙型肝炎病毒对人T细胞系的体外感染]

[In vitro infection of the human T-cell line with the hepatitis C virus].

作者信息

Ye J, Goeser T

机构信息

Dept. of Infection, Union Hospital of Tongji Medical University, Wuhan 430022.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 1997 Mar;11(1):41-3.

PMID:15619902

Abstract

A human T-cell line (Molt-4) was infected with an hepatitis C virus RNA (HCV- RNA) positive serum for 12 hours and incubated continously for 1-28 days. A productive infection was demonstrated by polymerase chain reaction amplification. Viral plus-stranded RNA was detectable in Molt-4 on the 1st, 2nd, 3rd day and the 8th day post infection. Viral minus-stranded RNA was detectable on the 2nd day and 8th day post infection.

摘要

用人丙型肝炎病毒RNA（HCV-RNA）阳性血清感染人T细胞系（Molt-4）12小时，然后连续培养1至28天。通过聚合酶链反应扩增证实存在有效感染。感染后第1天、第2天、第3天和第8天，在Molt-4细胞中可检测到病毒正链RNA。感染后第2天和第8天可检测到病毒负链RNA。