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[种间芥菜+拟南芥体细胞杂种的获得及转基因性状表现研究]

[Obtaining of intertribal Brassica juncea+Arabidopsis thaliana somatic hybrids and study of transgenic trait behaviour].

作者信息

Ovcharenko O O, Komarnyts'kyĭ I K, Cherep M M, Hleba Iu Iu, Kuchuk M V

出版信息

Tsitol Genet. 2004 May-Jun;38(3):3-8.

Abstract

Intertribal somatic hybridization between wild type Brassica juncea (L.) Czern. & Coss. and transgenic A. thaliana L. has been carried out. Genome of A. thaliana plants contained heterologous transposable element Spm/dSpm, reporter GUS gene, selective genes for kanamycin- (npt II) and phosphinothricin (bar) resistance. Hybrid nature of obtained plants was confirmed with their morphology, GUS hystochemical assay, PCR-RFLP, RAPD and isozyme analyses. It was determined that heterologous transposable element Spm/dSpm is able to function in hybrid plants. There was no complete elimination of A. thaliana genetic material in the hybrids and the transgenes were stably maintained.

摘要

已开展了野生型芥菜(Brassica juncea (L.) Czern. & Coss.)与转基因拟南芥(A. thaliana L.)之间的种间体细胞杂交。拟南芥植株的基因组包含异源转座因子Spm/dSpm、报告基因GUS、卡那霉素抗性(npt II)和草丁膦抗性(bar)的选择基因。通过形态学、GUS组织化学分析、PCR-RFLP、RAPD和同工酶分析,证实了所获植株的杂种性质。已确定异源转座因子Spm/dSpm能够在杂种植物中发挥作用。杂种中拟南芥的遗传物质并未完全消除,转基因得以稳定保留。

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